Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JHS83_RS13815 Genome accession   NZ_CP068622
Coordinates   2958036..2959052 (-) Length   338 a.a.
NCBI ID   WP_031778293.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain LH24     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2953036..2964052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHS83_RS13790 (JHS83_13765) aroK 2953896..2954414 (-) 519 WP_005381319.1 shikimate kinase AroK -
  JHS83_RS13795 (JHS83_13770) pilQ 2954603..2956351 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  JHS83_RS13800 (JHS83_13775) pilP 2956383..2956898 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  JHS83_RS13805 (JHS83_13780) pilO 2956888..2957481 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  JHS83_RS13810 (JHS83_13785) pilN 2957474..2958052 (-) 579 WP_140391938.1 PilN domain-containing protein Machinery gene
  JHS83_RS13815 (JHS83_13790) pilM 2958036..2959052 (-) 1017 WP_031778293.1 type IV pilus biogenesis protein PilM Machinery gene
  JHS83_RS13820 (JHS83_13795) - 2959195..2961765 (+) 2571 WP_140222517.1 penicillin-binding protein 1A -
  JHS83_RS13825 (JHS83_13800) oxyR 2961821..2962729 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  JHS83_RS13830 (JHS83_13805) - 2962900..2963628 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37820.32 Da        Isoelectric Point: 6.6637

>NTDB_id=530000 JHS83_RS13815 WP_031778293.1 2958036..2959052(-) (pilM) [Vibrio parahaemolyticus strain LH24]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDDQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=530000 JHS83_RS13815 WP_031778293.1 2958036..2959052(-) (pilM) [Vibrio parahaemolyticus strain LH24]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGATCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482