Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   K8B71_RS31695 Genome accession   NZ_CP082823
Coordinates   6584733..6585356 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6579733..6590356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B71_RS31675 (K8B71_31675) aroB 6580328..6581434 (-) 1107 WP_003135765.1 3-dehydroquinate synthase -
  K8B71_RS31680 (K8B71_31680) aroK 6581484..6582002 (-) 519 WP_003095833.1 shikimate kinase AroK -
  K8B71_RS31685 (K8B71_31685) pilQ 6582014..6584158 (-) 2145 WP_003135767.1 type 4a pilus secretin PilQ Machinery gene
  K8B71_RS31690 (K8B71_31690) comL/pilP 6584212..6584736 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  K8B71_RS31695 (K8B71_31695) pilO 6584733..6585356 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  K8B71_RS31700 (K8B71_31700) pilN 6585353..6585949 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  K8B71_RS31705 (K8B71_31705) pilM 6585949..6587013 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  K8B71_RS31710 (K8B71_31710) - 6587198..6589669 (+) 2472 WP_003135771.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=529958 K8B71_RS31695 WP_003103268.1 6584733..6585356(-) (pilO) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=529958 K8B71_RS31695 WP_003103268.1 6584733..6585356(-) (pilO) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTCC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401