NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	JKJ03_RS11780	Genome accession	NZ_CP068563
Coordinates	2447135..2448205 (-)	Length	356 a.a.
NCBI ID	WP_070082113.1	Uniprot ID	-
Organism	Bacillus velezensis strain GUMT319
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2442135..2453205

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JKJ03_RS11730 (JKJ03_11730)	sipW	2442195..2442779 (-)	585	WP_060562614.1	signal peptidase I SipW	-
JKJ03_RS11735 (JKJ03_11735)	tapA	2442751..2443422 (-)	672	WP_202735627.1	amyloid fiber anchoring/assembly protein TapA	-
JKJ03_RS11740 (JKJ03_11740)	-	2443681..2444010 (+)	330	WP_071391612.1	DUF3889 domain-containing protein	-
JKJ03_RS11745 (JKJ03_11745)	-	2444050..2444229 (-)	180	WP_003153093.1	YqzE family protein	-
JKJ03_RS11750 (JKJ03_11750)	comGG	2444286..2444663 (-)	378	WP_071391611.1	competence type IV pilus minor pilin ComGG	Machinery gene
JKJ03_RS11755 (JKJ03_11755)	comGF	2444664..2445059 (-)	396	WP_046559876.1	competence type IV pilus minor pilin ComGF	-
JKJ03_RS11760 (JKJ03_11760)	comGE	2445073..2445387 (-)	315	WP_015388003.1	competence type IV pilus minor pilin ComGE	Machinery gene
JKJ03_RS11765 (JKJ03_11765)	comGD	2445371..2445808 (-)	438	WP_025852922.1	competence type IV pilus minor pilin ComGD	Machinery gene
JKJ03_RS11770 (JKJ03_11770)	comGC	2445798..2446106 (-)	309	WP_003153087.1	competence type IV pilus major pilin ComGC	Machinery gene
JKJ03_RS11775 (JKJ03_11775)	comGB	2446111..2447148 (-)	1038	WP_063174751.1	competence type IV pilus assembly protein ComGB	Machinery gene
JKJ03_RS11780 (JKJ03_11780)	comGA	2447135..2448205 (-)	1071	WP_070082113.1	competence type IV pilus ATPase ComGA	Machinery gene
JKJ03_RS11785 (JKJ03_11785)	-	2448396..2449346 (-)	951	WP_071391609.1	magnesium transporter CorA family protein	-
JKJ03_RS11790 (JKJ03_11790)	-	2449492..2450793 (+)	1302	WP_070082114.1	hemolysin family protein	-
JKJ03_RS11795 (JKJ03_11795)	-	2450831..2451661 (-)	831	WP_071391608.1	STAS domain-containing protein	-
JKJ03_RS11805 (JKJ03_11805)	-	2451866..2452246 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
JKJ03_RS11810 (JKJ03_11810)	-	2452483..2452728 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40557.91 Da Isoelectric Point: 9.5862

                                >NTDB_id=529474 JKJ03_RS11780 WP_070082113.1 2447135..2448205(-) (comGA) [Bacillus velezensis strain GUMT319]

MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFREAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=529474 JKJ03_RS11780 WP_070082113.1 2447135..2448205(-) (comGA) [Bacillus velezensis strain GUMT319]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT

GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTCTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC

ACAAGGGATGCTAAGGGTGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC

CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTTACCCAATGCTTTCGGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	81.742	100	0.817
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0529474