Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   K8B92_RS19625 Genome accession   NZ_CP082776
Coordinates   4075240..4075899 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli O4:H5 strain CU532-9     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4070240..4080899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B92_RS19610 (K8B92_19525) ygiS 4072445..4074052 (-) 1608 WP_001536337.1 ABC transporter substrate-binding protein -
  K8B92_RS19615 (K8B92_19530) ygiV 4074161..4074643 (-) 483 WP_000183493.1 GyrI-like domain-containing protein -
  K8B92_RS19620 (K8B92_19535) ygiW 4074696..4075088 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  K8B92_RS19625 (K8B92_19540) ciaR 4075240..4075899 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  K8B92_RS19630 (K8B92_19545) qseC 4075896..4077245 (+) 1350 WP_000673388.1 quorum sensing histidine kinase QseC -
  K8B92_RS19635 (K8B92_19550) ygiZ 4077291..4077623 (-) 333 WP_000914696.1 DUF2645 family protein -
  K8B92_RS19640 - 4077630..4078340 (-) 711 WP_000834024.1 hypothetical protein -
  K8B92_RS19645 - 4078343..4078822 (-) 480 WP_000065329.1 Hcp family type VI secretion system effector -
  K8B92_RS19650 (K8B92_19560) mdaB 4079072..4079653 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  K8B92_RS19655 (K8B92_19565) ygiN 4079684..4079998 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=528978 K8B92_RS19625 WP_001221493.1 4075240..4075899(+) (ciaR) [Escherichia coli O4:H5 strain CU532-9]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=528978 K8B92_RS19625 WP_001221493.1 4075240..4075899(+) (ciaR) [Escherichia coli O4:H5 strain CU532-9]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAAGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365