Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   K8B93_RS20985 Genome accession   NZ_CP082772
Coordinates   4359978..4360637 (+) Length   219 a.a.
NCBI ID   WP_001221477.1    Uniprot ID   A0A0H2VCI3
Organism   Escherichia coli O2:H1 strain HM670     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4354978..4365637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B93_RS20970 (K8B93_20855) ygiS 4357183..4358790 (-) 1608 WP_001305104.1 ABC transporter substrate-binding protein -
  K8B93_RS20975 (K8B93_20860) ygiV 4358899..4359381 (-) 483 WP_000183488.1 GyrI-like domain-containing protein -
  K8B93_RS20980 (K8B93_20865) ygiW 4359434..4359826 (-) 393 WP_000712665.1 OB fold stress tolerance protein YgiW -
  K8B93_RS20985 (K8B93_20870) ciaR 4359978..4360637 (+) 660 WP_001221477.1 quorum sensing response regulator transcription factor QseB Regulator
  K8B93_RS20990 (K8B93_20875) qseC 4360634..4361983 (+) 1350 WP_001617272.1 quorum sensing histidine kinase QseC -
  K8B93_RS20995 (K8B93_20880) ygiZ 4362029..4362361 (-) 333 WP_000914696.1 DUF2645 family protein -
  K8B93_RS21000 - 4362368..4363078 (-) 711 WP_000834021.1 hypothetical protein -
  K8B93_RS21005 - 4363081..4363560 (-) 480 WP_000069056.1 Hcp family type VI secretion system effector -
  K8B93_RS21010 (K8B93_20890) mdaB 4363810..4364391 (+) 582 Protein_4102 NADPH:quinone oxidoreductase MdaB -
  K8B93_RS21015 (K8B93_20895) ygiN 4364422..4364736 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24756.74 Da        Isoelectric Point: 7.4218

>NTDB_id=528631 K8B93_RS20985 WP_001221477.1 4359978..4360637(+) (ciaR) [Escherichia coli O2:H1 strain HM670]
MRILLIEDDMLIGDGIKTGLSKMGFRVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=528631 K8B93_RS20985 WP_001221477.1 4359978..4360637(+) (ciaR) [Escherichia coli O2:H1 strain HM670]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTCGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TAGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VCI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365