Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   JHW37_RS24385 Genome accession   NZ_CP068238
Coordinates   5198448..5200040 (+) Length   530 a.a.
NCBI ID   WP_053817770.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain A39-1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5193448..5205040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW37_RS24365 (JHW37_24365) pgeF 5195201..5195929 (-) 729 WP_053817769.1 peptidoglycan editing factor PgeF -
  JHW37_RS24370 (JHW37_24370) rluD 5195926..5196888 (-) 963 WP_003150035.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  JHW37_RS24375 (JHW37_24375) - 5197034..5198059 (+) 1026 WP_012077305.1 outer membrane protein assembly factor BamD -
  JHW37_RS24380 (JHW37_24380) - 5198213..5198458 (+) 246 WP_003150030.1 PP0621 family protein -
  JHW37_RS24385 (JHW37_24385) pilS 5198448..5200040 (+) 1593 WP_053817770.1 two-component system sensor histidine kinase PilS Regulator
  JHW37_RS24390 (JHW37_24390) pilR 5200056..5201393 (+) 1338 WP_033999343.1 two-component system response regulator PilR Regulator
  JHW37_RS24395 (JHW37_24395) thiO 5201439..5202533 (-) 1095 WP_033999365.1 glycine oxidase ThiO -
  JHW37_RS24400 (JHW37_24400) - 5202668..5203177 (+) 510 WP_003150026.1 GspH/FimT family protein -
  JHW37_RS24405 (JHW37_24405) - 5203283..5203789 (+) 507 WP_012077308.1 Tfp pilus assembly protein FimT/FimU -
  JHW37_RS24410 (JHW37_24410) pilV 5203780..5204337 (+) 558 WP_034081446.1 type IV pilus modification protein PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59170.42 Da        Isoelectric Point: 7.1538

>NTDB_id=528426 JHW37_RS24385 WP_053817770.1 5198448..5200040(+) (pilS) [Pseudomonas aeruginosa strain A39-1]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEEQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLLLCGLFYAGGGMPSGIGSLLVVAVAIANILLRGRIGLVIAAVASLGLLYLTFFLSLSSPDAPNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRLSILLANQAALGLLRQDQVRGTSLD
RYSPMLVHCMKQWRLNPSRRPPTLKVIPEGPAVQPSFISLNREHDRHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDGADRRLTQIIQDQSRRMNLVIENVLQLSRRRQAEPQQLDLKDWLQRFVDEYPGKLRND
SELHLQLGAGDIHTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDADSDLPVLEIADDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=528426 JHW37_RS24385 WP_053817770.1 5198448..5200040(+) (pilS) [Pseudomonas aeruginosa strain A39-1]
GTGCGCGCTGAGCGGCTGCGGCTGAGCGAGGAGCAAGGGCAGCGCATCCTCCGCCTGTATCACCTGTATCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAGCTGGAAGAACAGGTCCTGAAACTGGTCCATCCCGAATTGTTCCATG
TCGGCAGCTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCACGGCAATTGCTGCCGATCTTC
ATCCTCGCCCTCACCGACGTGCTGCTGCTCTGCGGCCTGTTCTACGCCGGCGGCGGCATGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCGATCGCCAACATCCTCCTGCGCGGGCGCATCGGTCTGGTGATCGCGGCGGTGGCCAGCCTGG
GCCTGCTGTACCTGACCTTCTTCCTCAGCCTGAGCAGCCCGGACGCCCCCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTACGGCGCCAGGAACAGACGGAAACGCTGGCCGAGGAACG
CGCCGAGACGGTCGCCAACCTCGAAGAGCTCAACGCGCTGATCCTGCAGCGCATGCGCACCGGCATCCTGGTGGTCGACA
GCCGCCTGAGCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACCAGGTGCGGGGCACCAGCCTGGAC
CGCTACAGCCCGATGCTGGTGCACTGCATGAAGCAGTGGCGCCTCAACCCCAGTCGCCGCCCGCCGACCCTCAAGGTGAT
TCCGGAGGGCCCCGCCGTGCAGCCCAGCTTCATCAGCCTCAACCGTGAACACGACCGGCATGTGCTGATCTTCCTCGAAG
ACATCTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTCGCCGGCCTCGGCCGGCTGACCGCCGGCATCGCCCACGAG
ATCCGCAACCCGCTGGGCGCCATCAGCCACGCCGCGCAACTGCTGCAGGAGTCCGAGGAACTCGACGGCGCCGACCGCCG
CCTGACACAGATCATCCAGGACCAGTCGCGACGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGCCGCCGCC
AGGCCGAACCGCAGCAACTCGACCTGAAGGACTGGCTGCAACGCTTCGTCGACGAATATCCCGGCAAGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGCGCCGGCGACATCCACACGCGCATGGACCCGCACCAGTTGAATCAGGTACTGAGCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGTCTCGCGCGCGATGCGG
ACAGCGACCTGCCGGTGCTGGAAATCGCCGACGACGGCCCCGGCGTGCCGACGGACAAGCTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAATTGTGCGAGAGCAACCAGGCGCGCAT
CGACTACCGCAATCGCGAGGAGGGTGGAGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

94.528

100

0.945