Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6I50_RS09615 Genome accession   NZ_CP068195
Coordinates   1980425..1981546 (-) Length   373 a.a.
NCBI ID   WP_010326019.1    Uniprot ID   A0A1T1JGA2
Organism   Acinetobacter johnsonii strain FDAARGOS_1093     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1975425..1986546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I50_RS09585 (I6I50_09585) - 1976244..1977092 (+) 849 WP_184118212.1 class II glutamine amidotransferase -
  I6I50_RS09590 (I6I50_09590) - 1977367..1977822 (+) 456 WP_005400160.1 bacteriohemerythrin -
  I6I50_RS09595 (I6I50_09595) - 1977965..1979047 (+) 1083 WP_201706116.1 hypothetical protein -
  I6I50_RS09600 (I6I50_09600) - 1979047..1979370 (+) 324 WP_201706117.1 RnfH family protein -
  I6I50_RS09605 (I6I50_09605) - 1979417..1979815 (-) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  I6I50_RS09610 (I6I50_09610) fur 1979928..1980365 (+) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  I6I50_RS09615 (I6I50_09615) pilU 1980425..1981546 (-) 1122 WP_010326019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6I50_RS09620 (I6I50_09620) pilT 1981573..1982610 (-) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  I6I50_RS09625 (I6I50_09625) - 1982733..1983422 (+) 690 WP_125273868.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6I50_RS09630 (I6I50_09630) - 1983613..1984347 (+) 735 WP_005400153.1 hypothetical protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41863.14 Da        Isoelectric Point: 6.6664

>NTDB_id=527951 I6I50_RS09615 WP_010326019.1 1980425..1981546(-) (pilU) [Acinetobacter johnsonii strain FDAARGOS_1093]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHASANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=527951 I6I50_RS09615 WP_010326019.1 1980425..1981546(-) (pilU) [Acinetobacter johnsonii strain FDAARGOS_1093]
ATGGACTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCGGATTTATTTATCACCGCTGATGTTGAACCTTC
CATGAAAATTAACGGTCAAATTGTGCCTGTGGCTAAGGGTAAGCTGACGGGTGATGTCGCGGGGCAACTGCTCAACTCGA
TTATGAGTGAAAAACAGCGTAAAGAATTTGCAGATACACGTGAGTGCAACTTCGCGATTATGAATCGGGATAAAACTGCA
CGTTTTCGTGTCAGTGCATTTCAACAGCGTGATATGCCAGGTATGGTTTTGCGTCGGATTGAGACGCAAATCCCAACCAT
GGACGAGTTAAAACTTCCGCCTGTGCTTAAAGAGTTGGCCATGACCAAACGTGGCATCATCATCTTTGTGGGTGCAACAG
GTACGGGTAAATCGACCTCTTTGGCTTCGATCATTGGTTACCGTAACCAAAACTCAAAAGGTCATATCATTACCATTGAA
GACCCGATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACCCAGCGTGAAGTGGGCATTGATACAGACTCTTTTGA
AATCGCGCTGAAAAACACCTTACGTCAGGCACCCGATGTGATCCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCAACGCTGCACGCCAACAACGCCAACCAAGCCATTGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGCCAATTGTTTATGGACTTGTCTTTAAACCTTAAAGCGATGGTTGCTCA
GCAGTTGATTCCAACACCAGACGGTAATTCTCGTCGCGCTGCAATTGAGATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAACGTTCTCGTGAACTCGGTATGCAAACCTTTGACCAA
GCCTTATTCGATTTGTACAAAGCTAAGCAGATTACCTATAAAGATGCGCTGAAACACGCCGACTCTCCGAACGACTTACG
CTTACAAATCAAGTTGTCTGAAGAAGGCGGTGATCGTCTATTGCATGCCAGTGCAAACATCACGTTTGACGGCCAAGCTT
AA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.464

0.678
  pilU Vibrio cholerae strain A1552

55.135

99.196

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

92.761

0.394
  pilT Legionella pneumophila strain Lp02

41.124

90.617

0.373
  pilT Legionella pneumophila strain ERS1305867

41.124

90.617

0.373
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362