Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I6J10_RS05165 Genome accession   NZ_CP068061
Coordinates   1063259..1064161 (-) Length   300 a.a.
NCBI ID   WP_107537366.1    Uniprot ID   -
Organism   Mammaliicoccus vitulinus strain FDAARGOS_1153     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1058259..1069161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J10_RS05130 (I6J10_05130) gcvT 1058792..1059880 (-) 1089 WP_107537285.1 glycine cleavage system aminomethyltransferase GcvT -
  I6J10_RS05135 (I6J10_05135) - 1060073..1060561 (-) 489 WP_016911895.1 shikimate kinase -
  I6J10_RS05140 (I6J10_05140) comGF 1060796..1061230 (-) 435 WP_016911893.1 competence type IV pilus minor pilin ComGF -
  I6J10_RS05145 (I6J10_05145) comGE 1061205..1061486 (-) 282 WP_016911892.1 competence type IV pilus minor pilin ComGE -
  I6J10_RS05150 (I6J10_05150) comGD 1061470..1061898 (-) 429 WP_016911891.1 competence type IV pilus minor pilin ComGD -
  I6J10_RS05155 (I6J10_05155) comGC 1061898..1062215 (-) 318 WP_016911890.1 competence type IV pilus major pilin ComGC Machinery gene
  I6J10_RS05160 (I6J10_05160) comGB 1062229..1063266 (-) 1038 WP_119595883.1 competence type IV pilus assembly protein ComGB -
  I6J10_RS05165 (I6J10_05165) comGA 1063259..1064161 (-) 903 WP_107537366.1 competence type IV pilus ATPase ComGA Machinery gene
  I6J10_RS05170 (I6J10_05170) - 1064315..1064560 (+) 246 WP_016911887.1 DUF2626 family protein -
  I6J10_RS05175 (I6J10_05175) - 1064597..1065229 (-) 633 WP_016911886.1 MBL fold metallo-hydrolase -
  I6J10_RS05180 (I6J10_05180) - 1065329..1065568 (+) 240 WP_016911885.1 DUF2759 domain-containing protein -
  I6J10_RS05185 (I6J10_05185) - 1065598..1065912 (-) 315 WP_016911884.1 MTH1187 family thiamine-binding protein -
  I6J10_RS05190 (I6J10_05190) - 1065912..1066883 (-) 972 WP_016911883.1 ROK family glucokinase -
  I6J10_RS05195 (I6J10_05195) - 1066880..1067071 (-) 192 WP_016911882.1 YqgQ family protein -
  I6J10_RS05200 (I6J10_05200) - 1067075..1068523 (-) 1449 WP_016911881.1 rhomboid family intramembrane serine protease -
  I6J10_RS05205 (I6J10_05205) - 1068524..1069078 (-) 555 WP_103323127.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34605.82 Da        Isoelectric Point: 6.4113

>NTDB_id=526840 I6J10_RS05165 WP_107537366.1 1063259..1064161(-) (comGA) [Mammaliicoccus vitulinus strain FDAARGOS_1153]
MEKLFKSIIEDAIKSNVSDIHFIPVIDDKIVVQMRFNGDNKDYDLSLDAKLYNRLLIYMKFISHLDVSERNKAQSGQCIY
EHVMNYYLRISTIPISLGIESCAIRITPQYFIESDANRLNSDFIEIRNKMYQSEGLFLFTGPTGSGKTTLMYELMYDLKK
EKNKHIITIEDPVEQTLNGVVQVSVNERANISYQQSFKAILRCDPDVIMIGEIRDEETAKQVIQASLSGHLVLSTMHAKD
NIGAIHRLIELGITVEQIKQGISCIANQRLVKQDESSRCRKMSYMFSEHIIQYMVRDYHE

Nucleotide


Download         Length: 903 bp        

>NTDB_id=526840 I6J10_RS05165 WP_107537366.1 1063259..1064161(-) (comGA) [Mammaliicoccus vitulinus strain FDAARGOS_1153]
ATGGAGAAATTATTTAAATCAATTATCGAAGACGCTATAAAATCAAATGTAAGCGATATTCATTTCATACCTGTAATAGA
CGATAAGATTGTTGTTCAGATGAGATTTAATGGTGACAACAAAGACTATGATTTATCGCTAGATGCTAAATTGTATAACA
GATTGCTAATCTATATGAAATTTATTTCTCACTTAGACGTAAGTGAGAGAAACAAAGCACAAAGTGGTCAGTGTATTTAT
GAACATGTTATGAATTATTATTTGAGAATTTCGACGATACCAATTAGTTTAGGGATAGAATCATGTGCAATCAGGATAAC
ACCTCAATATTTTATTGAAAGTGATGCAAATAGGCTTAATTCAGATTTTATAGAAATTAGAAATAAGATGTATCAATCTG
AAGGTTTATTTCTATTTACTGGGCCTACAGGATCTGGTAAGACAACTTTAATGTATGAATTAATGTATGACTTAAAGAAA
GAAAAGAATAAACATATTATTACGATTGAGGATCCTGTGGAACAAACATTAAATGGCGTTGTACAAGTTTCTGTTAATGA
AAGAGCGAATATATCTTATCAACAGTCATTTAAAGCAATTTTAAGATGTGATCCTGATGTTATTATGATAGGTGAAATAA
GAGATGAAGAAACTGCTAAGCAAGTTATACAAGCAAGTTTAAGTGGGCATCTTGTGCTTTCAACTATGCATGCTAAAGAT
AATATCGGTGCAATACATAGATTAATTGAATTAGGCATAACTGTAGAACAAATTAAGCAAGGCATATCGTGTATTGCAAA
TCAGCGATTAGTTAAGCAAGATGAATCAAGTCGCTGTCGAAAAATGAGTTACATGTTTAGTGAACATATCATTCAATATA
TGGTGCGTGATTATCATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.495

99

0.49
  comGA Staphylococcus aureus N315

49.495

99

0.49
  comGA Bacillus subtilis subsp. subtilis str. 168

42.238

92.333

0.39