Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   B657_RS13845 Genome accession   NC_018520
Coordinates   2619985..2620602 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis QB928     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2614985..2625602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B657_RS13820 rpsT 2615287..2615553 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  B657_RS13825 holA 2615568..2616611 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  B657_RS22495 - 2616651..2616800 (-) 150 WP_003229985.1 hypothetical protein -
  B657_RS13830 yqzM 2616841..2616975 (+) 135 WP_003229983.1 YqzM family protein -
  B657_RS13835 comEC 2617015..2619345 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  B657_RS13840 comEB 2619349..2619918 (-) 570 WP_003229978.1 ComE operon protein 2 -
  B657_RS13845 comEA 2619985..2620602 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  B657_RS13850 comER 2620686..2621507 (+) 822 WP_004398597.1 late competence protein ComER -
  B657_RS13855 yqeM 2621573..2622316 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  B657_RS13860 rsfS 2622313..2622669 (-) 357 WP_003229971.1 ribosome silencing factor -
  B657_RS13865 yqeK 2622687..2623247 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  B657_RS13870 nadD 2623237..2623806 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  B657_RS13875 yhbY 2623818..2624108 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  B657_RS13880 aroE 2624102..2624944 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=52614 B657_RS13845 WP_004398514.1 2619985..2620602(-) (comEA) [Bacillus subtilis QB928]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=52614 B657_RS13845 WP_004398514.1 2619985..2620602(-) (comEA) [Bacillus subtilis QB928]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment