Detailed information NTG0524471

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JFY49_RS08145
• Genome accession: NZ_CP067019
• Coordinates: 1618476..1619672 (+)
• Length: 398 a.a.
• NCBI ID: WP_200224762.1
• Uniprot ID: A0A7T7R9M8
• Organism: Acinetobacter sp. CS-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1613476..1624672

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JFY49_RS08130 (JFY49_08130)	-	1614458..1615663 (-)	1206	WP_180082198.1	aromatic amino acid transaminase	-
JFY49_RS08135 (JFY49_08135)	-	1615813..1616514 (-)	702	WP_001223318.1	IS1-like element ISPa14 family transposase	-
JFY49_RS08140 (JFY49_08140)	-	1617082..1618143 (+)	1062	WP_180042584.1	3-deoxy-7-phosphoheptulonate synthase	-
JFY49_RS08145 (JFY49_08145)	pilU	1618476..1619672 (+)	1197	WP_200224762.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
JFY49_RS08150 (JFY49_08150)	gcvH	1619828..1620202 (-)	375	WP_200224764.1	glycine cleavage system protein GcvH	-
JFY49_RS08155 (JFY49_08155)	-	1620274..1621302 (-)	1029	WP_180042582.1	helix-turn-helix transcriptional regulator	-
JFY49_RS08160 (JFY49_08160)	-	1621411..1621860 (-)	450	WP_200224765.1	MaoC family dehydratase	-
JFY49_RS08165 (JFY49_08165)	-	1621860..1623713 (-)	1854	WP_200224766.1	ABC transporter ATP-binding protein	-
JFY49_RS08170 (JFY49_08170)	-	1623915..1624177 (-)	263	Protein_1587	IS5/IS1182 family transposase	-

Sequence

Protein

• Length: 398 a.a.
• Molecular weight: 44974.47 Da
• Isoelectric Point: 6.7140

>NTDB_id=524471 JFY49_RS08145 WP_200224762.1 1618476..1619672(+) (pilU) [Acinetobacter sp. CS-2]
MYSTELLEEARKMMFHMLTKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQALTADKTKLFAYSLMNEKQRHEFETELEC
NFAISVPNVSRFRVNVFQQQLHVGMVIRTITAEIPNFDQLKLPESLKHVMMAKRGLILVVGATGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRAAIEILLNTPLIGDNILKGQFHELKEIMKKS
RELGMQTFDQALYDLYNEGAISYEEALRNADSMNELRLQIKLKSNRQDQTVPGSAASFSMLQDKDPEENQAKIGPETP

Nucleotide

• Length: 1197 bp

>NTDB_id=524471 JFY49_RS08145 WP_200224762.1 1618476..1619672(+) (pilU) [Acinetobacter sp. CS-2]
ATGTATAGTACGGAATTACTGGAAGAAGCACGTAAAATGATGTTCCATATGCTGACTAAGGTGGTGGAATATGGCGGCTC
AGATTTATTTATTTCAGCAGATTTTCCACCGAGTATTAAACATCAGGGCTTAATGAAACCGCTTGGCCAGCAGGCATTGA
CTGCCGACAAGACCAAGTTGTTTGCCTATAGTTTAATGAATGAAAAACAGAGGCATGAGTTTGAAACCGAGCTGGAATGT
AATTTTGCCATTAGTGTTCCCAATGTTTCGCGCTTTCGTGTCAATGTATTCCAGCAGCAATTACATGTAGGGATGGTAAT
ACGAACCATTACCGCAGAAATTCCAAATTTTGACCAGTTAAAGCTTCCCGAGTCCTTAAAGCATGTGATGATGGCAAAAC
GCGGTCTGATACTTGTGGTCGGTGCTACCGGCTCGGGTAAATCGACTTCTTTAGCCGCCATGATTGATCATCGTAATGAA
AATTCTGCCGGGCACATTATTACCGTGGAAGATCCGGTGGAATATGTGCATAAGCATAAGAAATCGATGATTACCCACCG
CGAAGTCGGCGTGGATAGTCATTCATGGCACAATGCCTTGAAGAACACCTTGCGTCAGGCACCGGATGTTATTTTAATTG
GTGAAATCCGTGATACCGAAACCATGGAACATGCCATTGCCTTTGCCGAAACCGGACATTTATGTTTAGGTACTTTACAT
GCCAATAATGCCAATCAGGCGCTGGATCGAATTATCAACTTTTTCCCGGAAGAACGTCGCAATCAATTGCTGATGGATTT
ATCTTCCAATATGAAGGCGATTATTTCACAGCGTTTGGTGCGTACTGAAGATGGACGTGGACGTCGTGCAGCCATTGAAA
TTTTGTTGAATACACCTTTAATTGGAGATAATATCCTGAAAGGCCAGTTTCATGAACTCAAAGAAATCATGAAGAAATCC
CGTGAATTGGGCATGCAAACTTTCGATCAGGCTTTATATGATTTATATAATGAGGGTGCGATCAGTTATGAAGAAGCATT
GCGTAATGCCGACTCCATGAATGAATTGCGCTTGCAGATTAAATTGAAAAGTAACCGACAGGATCAAACGGTTCCAGGTT
CAGCTGCATCATTCAGTATGCTGCAAGATAAAGATCCGGAAGAGAATCAAGCTAAGATAGGACCGGAAACACCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (105-282)

3D structure

• AlphaFold DB: A0A7T7R9M8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.221	93.216	0.543
pilU	Acinetobacter baylyi ADP1	56.484	87.186	0.492
pilU	Vibrio cholerae strain A1552	52.101	89.698	0.467