Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   JFJ84_RS03135 Genome accession   NZ_CP066739
Coordinates   614493..615032 (+) Length   179 a.a.
NCBI ID   WP_012341221.1    Uniprot ID   -
Organism   Histophilus somni strain ASc-MMNZ-VFA-069     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 609493..620032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFJ84_RS03115 (JFJ84_03110) lpxM 609494..610447 (+) 954 WP_012341217.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
  JFJ84_RS03120 (JFJ84_03115) apt 610518..611057 (+) 540 WP_198974110.1 adenine phosphoribosyltransferase -
  JFJ84_RS03125 (JFJ84_03120) dnaX 611088..613091 (+) 2004 WP_012341219.1 DNA polymerase III subunit gamma/tau -
  JFJ84_RS03130 (JFJ84_03125) - 613344..614462 (+) 1119 WP_012341220.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  JFJ84_RS03135 (JFJ84_03130) pilF/pilF2 614493..615032 (+) 540 WP_012341221.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  JFJ84_RS03140 (JFJ84_03135) nrdA 615115..617385 (-) 2271 WP_012341222.1 class 1a ribonucleoside-diphosphate reductase subunit alpha -
  JFJ84_RS03145 (JFJ84_03140) - 617876..618133 (-) 258 WP_075294154.1 helix-turn-helix domain-containing protein -
  JFJ84_RS03150 (JFJ84_03145) - 618242..618457 (+) 216 WP_075294140.1 transposase -
  JFJ84_RS03155 (JFJ84_03150) - 618457..618603 (+) 147 WP_157665520.1 hypothetical protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20670.59 Da        Isoelectric Point: 9.3139

>NTDB_id=522203 JFJ84_RS03135 WP_012341221.1 614493..615032(+) (pilF/pilF2) [Histophilus somni strain ASc-MMNZ-VFA-069]
MRYRTIFTFIIFSLFLSACVSQPTTGRFNKQQAAKARIELGLGYLKLRNFSQAKLNFDKALFYQPDYYLVHSAFAYLYQL
QGDISQAKSSYEKAISLDKSQGDVKNNYGAFLCSQGEFARAYELFEQALTTPNYYQQADTYANIIICAEAEHNIPRYQKF
LQLLKNIDPKRAEEIKKVS

Nucleotide


Download         Length: 540 bp        

>NTDB_id=522203 JFJ84_RS03135 WP_012341221.1 614493..615032(+) (pilF/pilF2) [Histophilus somni strain ASc-MMNZ-VFA-069]
ATGCGATATCGGACTATATTTACTTTTATTATATTCTCTCTTTTTCTTTCTGCTTGCGTTTCTCAGCCTACAACTGGTCG
CTTTAATAAGCAACAAGCTGCAAAAGCACGAATTGAGTTAGGTTTAGGTTATTTAAAATTACGCAATTTTTCTCAAGCAA
AACTTAATTTTGATAAAGCTTTATTCTATCAACCTGACTATTATTTGGTACATTCGGCTTTTGCCTATTTATATCAATTA
CAAGGTGATATTTCGCAAGCGAAATCCTCTTATGAAAAAGCCATCAGTTTGGATAAGTCGCAAGGTGATGTGAAAAATAA
TTATGGTGCATTTTTATGTAGTCAGGGCGAATTTGCTCGTGCTTATGAGTTATTTGAGCAGGCACTTACTACGCCTAATT
ATTATCAGCAAGCGGATACCTATGCAAATATCATAATTTGTGCAGAGGCGGAACATAATATTCCACGTTATCAGAAATTT
TTACAGTTACTGAAGAATATTGATCCCAAAAGAGCGGAGGAAATAAAGAAAGTTTCTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_7)

(109-130)

Tetratricopeptide repeat (TPR_8)

(74-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.121

92.179

0.48
  pilF Glaesserella parasuis strain SC1401

44.068

98.883

0.436