Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   K3176_RS30120 Genome accession   NZ_CP081202
Coordinates   6265429..6266052 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain P9W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6260429..6271052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3176_RS30105 (K3176_30105) - 6261116..6263587 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  K3176_RS30110 (K3176_30110) pilM 6263772..6264836 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  K3176_RS30115 (K3176_30115) pilN 6264836..6265432 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  K3176_RS30120 (K3176_30120) pilO 6265429..6266052 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  K3176_RS30125 (K3176_30125) comL/pilP 6266049..6266573 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  K3176_RS30130 (K3176_30130) pilQ 6266627..6268771 (+) 2145 WP_003161249.1 type 4a pilus secretin PilQ Machinery gene
  K3176_RS30135 (K3176_30135) aroK 6268783..6269301 (+) 519 WP_003095833.1 shikimate kinase AroK -
  K3176_RS30140 (K3176_30140) aroB 6269351..6270457 (+) 1107 WP_023912813.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=521918 K3176_RS30120 WP_003103268.1 6265429..6266052(+) (pilO) [Pseudomonas aeruginosa strain P9W]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=521918 K3176_RS30120 WP_003103268.1 6265429..6266052(+) (pilO) [Pseudomonas aeruginosa strain P9W]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401