Detailed information NTG0052130

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LPO_RS07200
• Genome accession: NC_018139
• Coordinates: 1575097..1575960 (+)
• Length: 287 a.a.
• NCBI ID: WP_014841651.1
• Uniprot ID: I7HJU7
• Organism: Legionella pneumophila subsp. pneumophila
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1570097..1580960

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LPO_RS07180 (LPO_1533)	-	1570591..1570917 (-)	327	WP_011213802.1	DUF1820 family protein	-
LPO_RS07185 (LPO_1534)	-	1571100..1571843 (-)	744	WP_014841649.1	methyltransferase domain-containing protein	-
LPO_RS07190 (LPO_1535)	pilB	1572102..1573826 (+)	1725	WP_010947251.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LPO_RS07195 (LPO_1536)	pilC	1573832..1575052 (+)	1221	WP_014841650.1	type II secretion system F family protein	Machinery gene
LPO_RS07200 (LPO_1537)	pilD	1575097..1575960 (+)	864	WP_014841651.1	prepilin peptidase	Machinery gene
LPO_RS07205 (LPO_1538)	-	1575974..1577179 (-)	1206	WP_014841652.1	cyclic nucleotide-binding domain-containing protein	-
LPO_RS07210 (LPO_1539)	-	1577287..1577880 (-)	594	WP_011213808.1	hypothetical protein	-
LPO_RS07215 (LPO_1540)	-	1577896..1578351 (-)	456	WP_014841654.1	hypothetical protein	-
LPO_RS07220 (LPO_1541)	-	1578485..1579300 (+)	816	WP_014841655.1	pyruvate, water dikinase regulatory protein	-
LPO_RS07225 (LPO_1542)	-	1579420..1580868 (-)	1449	WP_014841656.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32756.36 Da
• Isoelectric Point: 7.8442

>NTDB_id=52130 LPO_RS07200 WP_014841651.1 1575097..1575960(+) (pilD) [Legionella pneumophila subsp. pneumophila]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIITWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=52130 LPO_RS07200 WP_014841651.1 1575097..1575960(+) (pilD) [Legionella pneumophila subsp. pneumophila]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCCGTTGGAAGTTT
GCTTAATGTGATAATCTATCGCTTGCCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGTCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGTGATAGTCCAATTTCAATCCGCTA
CCCTTTTGTTGAGACGTTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAATTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATGTATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCCTTATGGTTATTCATTAATCTGTTTTATCTGATGACAGGTAAGGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACGGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCAATTATCACTTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: I7HJU7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.11	97.909	0.481
pilD	Acinetobacter nosocomialis M2	47.985	95.122	0.456
pilD	Acinetobacter baumannii D1279779	47.273	95.819	0.453
pilD	Vibrio campbellii strain DS40M4	44.964	96.864	0.436
pilD	Neisseria gonorrhoeae MS11	42.446	96.864	0.411