Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JEQ25_RS13185 Genome accession   NZ_CP066380
Coordinates   2509713..2510783 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain ID-A05     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2504713..2515783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JEQ25_RS13140 (JEQ25_13060) tapA 2505191..2505952 (-) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -
  JEQ25_RS13145 (JEQ25_13065) yqzG 2506224..2506550 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  JEQ25_RS13150 (JEQ25_13070) spoIIT 2506592..2506771 (-) 180 WP_029726723.1 YqzE family protein -
  JEQ25_RS13155 (JEQ25_13075) comGG 2506843..2507217 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  JEQ25_RS13160 (JEQ25_13080) comGF 2507218..2507601 (-) 384 WP_046160582.1 ComG operon protein ComGF Machinery gene
  JEQ25_RS13165 (JEQ25_13085) comGE 2507627..2507974 (-) 348 WP_046381178.1 ComG operon protein 5 Machinery gene
  JEQ25_RS13170 (JEQ25_13090) comGD 2507958..2508389 (-) 432 WP_046381179.1 comG operon protein ComGD Machinery gene
  JEQ25_RS13175 (JEQ25_13095) comGC 2508379..2508675 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  JEQ25_RS13180 (JEQ25_13100) comGB 2508689..2509726 (-) 1038 WP_029317912.1 comG operon protein ComGB Machinery gene
  JEQ25_RS13185 (JEQ25_13105) comGA 2509713..2510783 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  JEQ25_RS13190 (JEQ25_13110) - 2510995..2511192 (-) 198 WP_014480259.1 CBS domain-containing protein -
  JEQ25_RS13195 (JEQ25_13115) corA 2511194..2512147 (-) 954 WP_004399136.1 magnesium transporter CorA -
  JEQ25_RS13200 (JEQ25_13120) yqhB 2512290..2513618 (+) 1329 WP_003230150.1 hemolysin family protein -
  JEQ25_RS13205 (JEQ25_13125) rsbRD 2513671..2514507 (-) 837 WP_046381180.1 RsbT co-antagonist protein RsbRD -
  JEQ25_RS13215 (JEQ25_13135) mgsR 2514731..2515111 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  JEQ25_RS13220 (JEQ25_13140) yqgY 2515343..2515588 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=519833 JEQ25_RS13185 WP_004399124.1 2509713..2510783(-) (comGA) [Bacillus subtilis strain ID-A05]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=519833 JEQ25_RS13185 WP_004399124.1 2509713..2510783(-) (comGA) [Bacillus subtilis strain ID-A05]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1