NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	JEQ16_RS12300	Genome accession	NZ_CP066377
Coordinates	2528631..2529245 (-)	Length	204 a.a.
NCBI ID	WP_013352942.1	Uniprot ID	A0A411A874
Organism	Bacillus velezensis strain ID-A01
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2523631..2534245

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JEQ16_RS12270 (JEQ16_12210)	rpsT	2523931..2524197 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JEQ16_RS12275 (JEQ16_12215)	holA	2524214..2525255 (-)	1042	Protein_2374	DNA polymerase III subunit delta	-
JEQ16_RS12280 (JEQ16_12220)	-	2525295..2525449 (-)	155	Protein_2375	hypothetical protein	-
JEQ16_RS12285 (JEQ16_12225)	-	2525490..2525624 (+)	135	WP_003152870.1	YqzM family protein	-
JEQ16_RS12290 (JEQ16_12230)	comEC	2525643..2527994 (-)	2352	WP_278071180.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JEQ16_RS12295 (JEQ16_12235)	-	2527995..2528564 (-)	570	WP_003152868.1	ComE operon protein 2	-
JEQ16_RS12300 (JEQ16_12240)	comEA	2528631..2529245 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
JEQ16_RS12305 (JEQ16_12245)	comER	2529304..2530125 (+)	822	WP_014305449.1	late competence protein ComER	-
JEQ16_RS12310 (JEQ16_12250)	-	2530194..2530931 (-)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
JEQ16_RS12315 (JEQ16_12255)	rsfS	2530928..2531284 (-)	357	WP_003152864.1	ribosome silencing factor	-
JEQ16_RS12320 (JEQ16_12260)	yqeK	2531302..2531862 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JEQ16_RS12325 (JEQ16_12265)	-	2531852..2532421 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
JEQ16_RS12330 (JEQ16_12270)	yhbY	2532432..2532722 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
JEQ16_RS12335 (JEQ16_12275)	aroE	2532716..2533558 (-)	843	WP_278071181.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22190.27 Da Isoelectric Point: 5.4841

                                >NTDB_id=519604 JEQ16_RS12300 WP_013352942.1 2528631..2529245(-) (comEA) [Bacillus velezensis strain ID-A01]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=519604 JEQ16_RS12300 WP_013352942.1 2528631..2529245(-) (comEA) [Bacillus velezensis strain ID-A01]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0519604