Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   JFU10_RS03640 Genome accession   NZ_CP066337
Coordinates   829979..830593 (-) Length   204 a.a.
NCBI ID   WP_013352942.1    Uniprot ID   A0A411A874
Organism   Bacillus velezensis strain Mr12     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 824979..835593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFU10_RS03610 rpsT 825279..825545 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  JFU10_RS03615 holA 825562..826603 (-) 1042 Protein_739 DNA polymerase III subunit delta -
  JFU10_RS03620 - 826643..826797 (-) 155 Protein_740 hypothetical protein -
  JFU10_RS03625 - 826838..826972 (+) 135 WP_003152870.1 YqzM family protein -
  JFU10_RS03630 comEC 826991..829342 (-) 2352 WP_198863809.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  JFU10_RS03635 - 829343..829912 (-) 570 WP_003152868.1 ComE operon protein 2 -
  JFU10_RS03640 comEA 829979..830593 (-) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  JFU10_RS03645 comER 830651..831472 (+) 822 WP_198863810.1 late competence protein ComER -
  JFU10_RS03650 - 831541..832278 (-) 738 WP_031378935.1 class I SAM-dependent DNA methyltransferase -
  JFU10_RS03655 rsfS 832275..832631 (-) 357 WP_003152864.1 ribosome silencing factor -
  JFU10_RS03660 yqeK 832649..833209 (-) 561 WP_031378934.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  JFU10_RS03665 - 833199..833768 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  JFU10_RS03670 yhbY 833779..834069 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  JFU10_RS03675 aroE 834063..834905 (-) 843 WP_031378933.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22190.27 Da        Isoelectric Point: 5.4841

>NTDB_id=519304 JFU10_RS03640 WP_013352942.1 829979..830593(-) (comEA) [Bacillus velezensis strain Mr12]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=519304 JFU10_RS03640 WP_013352942.1 829979..830593(-) (comEA) [Bacillus velezensis strain Mr12]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363