Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H9K57_RS14375 Genome accession   NZ_CP066246
Coordinates   2998437..2999030 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain DHO76     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2993437..3004030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K57_RS14355 (H9K57_14250) aroB 2994313..2995413 (-) 1101 WP_005480443.1 3-dehydroquinate synthase -
  H9K57_RS14360 (H9K57_14255) aroK 2995445..2995963 (-) 519 WP_005381319.1 shikimate kinase AroK -
  H9K57_RS14365 (H9K57_14260) pilQ 2996152..2997900 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  H9K57_RS14370 (H9K57_14265) pilP 2997932..2998447 (-) 516 WP_021453866.1 pilus assembly protein PilP Machinery gene
  H9K57_RS14375 (H9K57_14270) pilO 2998437..2999030 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  H9K57_RS14380 (H9K57_14275) pilN 2999023..2999601 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  H9K57_RS14385 (H9K57_14280) pilM 2999585..3000601 (-) 1017 WP_005496568.1 type IV pilus assembly protein PilM Machinery gene
  H9K57_RS14390 (H9K57_14285) - 3000744..3003314 (+) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=518900 H9K57_RS14375 WP_005480482.1 2998437..2999030(-) (pilO) [Vibrio parahaemolyticus strain DHO76]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=518900 H9K57_RS14375 WP_005480482.1 2998437..2999030(-) (pilO) [Vibrio parahaemolyticus strain DHO76]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATTTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726