Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   H9K57_RS14385 Genome accession   NZ_CP066246
Coordinates   2999585..3000601 (-) Length   338 a.a.
NCBI ID   WP_005496568.1    Uniprot ID   A0A3E1IL27
Organism   Vibrio parahaemolyticus strain DHO76     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2994585..3005601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K57_RS14360 (H9K57_14255) aroK 2995445..2995963 (-) 519 WP_005381319.1 shikimate kinase AroK -
  H9K57_RS14365 (H9K57_14260) pilQ 2996152..2997900 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  H9K57_RS14370 (H9K57_14265) pilP 2997932..2998447 (-) 516 WP_021453866.1 pilus assembly protein PilP Machinery gene
  H9K57_RS14375 (H9K57_14270) pilO 2998437..2999030 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  H9K57_RS14380 (H9K57_14275) pilN 2999023..2999601 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  H9K57_RS14385 (H9K57_14280) pilM 2999585..3000601 (-) 1017 WP_005496568.1 type IV pilus assembly protein PilM Machinery gene
  H9K57_RS14390 (H9K57_14285) - 3000744..3003314 (+) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  H9K57_RS14395 (H9K57_14290) oxyR 3003370..3004278 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  H9K57_RS14400 (H9K57_14295) - 3004449..3005177 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8323

>NTDB_id=518902 H9K57_RS14385 WP_005496568.1 2999585..3000601(-) (pilM) [Vibrio parahaemolyticus strain DHO76]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=518902 H9K57_RS14385 WP_005496568.1 2999585..3000601(-) (pilM) [Vibrio parahaemolyticus strain DHO76]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTAGGATTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1IL27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

85.207

100

0.852
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482