Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   JD965_RS12680 Genome accession   NZ_CP066219
Coordinates   2503008..2503622 (-) Length   204 a.a.
NCBI ID   WP_198697905.1    Uniprot ID   -
Organism   Bacillus siamensis strain B28     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2498008..2508622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JD965_RS12650 (JD965_12650) rpsT 2498308..2498574 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  JD965_RS12655 (JD965_12655) holA 2498592..2499633 (-) 1042 Protein_2470 DNA polymerase III subunit delta -
  JD965_RS12660 (JD965_12660) - 2499673..2499827 (-) 155 Protein_2471 hypothetical protein -
  JD965_RS12665 (JD965_12665) - 2499868..2500002 (+) 135 WP_003152870.1 YqzM family protein -
  JD965_RS12670 (JD965_12670) comEC 2500021..2502372 (-) 2352 WP_198697904.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  JD965_RS12675 (JD965_12675) - 2502373..2502942 (-) 570 WP_016938895.1 ComE operon protein 2 -
  JD965_RS12680 (JD965_12680) comEA 2503008..2503622 (-) 615 WP_198697905.1 helix-hairpin-helix domain-containing protein Machinery gene
  JD965_RS12685 (JD965_12685) comER 2503681..2504502 (+) 822 WP_198697906.1 late competence protein ComER -
  JD965_RS12690 (JD965_12690) - 2504571..2505308 (-) 738 WP_198697907.1 class I SAM-dependent DNA methyltransferase -
  JD965_RS12695 (JD965_12695) rsfS 2505305..2505661 (-) 357 WP_016938891.1 ribosome silencing factor -
  JD965_RS12700 (JD965_12700) yqeK 2505678..2506238 (-) 561 WP_198697908.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  JD965_RS12705 (JD965_12705) - 2506228..2506797 (-) 570 WP_045926759.1 nicotinate-nucleotide adenylyltransferase -
  JD965_RS12710 (JD965_12710) yhbY 2506808..2507098 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  JD965_RS12715 (JD965_12715) aroE 2507092..2507934 (-) 843 WP_198697909.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22280.30 Da        Isoelectric Point: 4.6994

>NTDB_id=518519 JD965_RS12680 WP_198697905.1 2503008..2503622(-) (comEA) [Bacillus siamensis strain B28]
MTKAMMTEQLRRNLKILGVCLAAGAIFICIWLFRSEKEEPVKQQSMFGQTASASGTDKTDTADDKIMIDMKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKEKADELNVNLAEQLQDGAVVYIPSEGEEENRPKTAAGEKEDAPVNINTATLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=518519 JD965_RS12680 WP_198697905.1 2503008..2503622(-) (comEA) [Bacillus siamensis strain B28]
ATGACGAAAGCGATGATGACGGAGCAGCTGCGGCGGAACCTGAAAATTCTGGGTGTTTGTCTTGCGGCAGGAGCGATTTT
CATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCGATGTTCGGCCAAACAGCCTCAGCAT
CCGGCACGGACAAAACAGACACCGCAGACGATAAGATTATGATTGATATGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAGGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAAGACGGGGCGGTCGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACCG
CAGCAGGTGAAAAGGAAGATGCCCCTGTCAATATCAATACGGCAACTCTGGACGAACTTCAGGCGATTTCCGGTGTGGGA
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGGGAAGAAAACGGACGGTTTCAGACGGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

56.716

98.529

0.559
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.749

100

0.363