Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   JD965_RS12670 Genome accession   NZ_CP066219
Coordinates   2500021..2502372 (-) Length   783 a.a.
NCBI ID   WP_198697904.1    Uniprot ID   -
Organism   Bacillus siamensis strain B28     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2495021..2507372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JD965_RS12635 (JD965_12635) - 2495383..2495721 (-) 339 WP_016938900.1 YqxA family protein -
  JD965_RS12640 (JD965_12640) spoIIP 2495738..2496931 (-) 1194 WP_198697902.1 stage II sporulation protein P -
  JD965_RS12645 (JD965_12645) gpr 2496999..2498105 (-) 1107 WP_095241591.1 GPR endopeptidase -
  JD965_RS12650 (JD965_12650) rpsT 2498308..2498574 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  JD965_RS12655 (JD965_12655) holA 2498592..2499633 (-) 1042 Protein_2470 DNA polymerase III subunit delta -
  JD965_RS12660 (JD965_12660) - 2499673..2499827 (-) 155 Protein_2471 hypothetical protein -
  JD965_RS12665 (JD965_12665) - 2499868..2500002 (+) 135 WP_003152870.1 YqzM family protein -
  JD965_RS12670 (JD965_12670) comEC 2500021..2502372 (-) 2352 WP_198697904.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  JD965_RS12675 (JD965_12675) - 2502373..2502942 (-) 570 WP_016938895.1 ComE operon protein 2 -
  JD965_RS12680 (JD965_12680) comEA 2503008..2503622 (-) 615 WP_198697905.1 helix-hairpin-helix domain-containing protein Machinery gene
  JD965_RS12685 (JD965_12685) comER 2503681..2504502 (+) 822 WP_198697906.1 late competence protein ComER -
  JD965_RS12690 (JD965_12690) - 2504571..2505308 (-) 738 WP_198697907.1 class I SAM-dependent DNA methyltransferase -
  JD965_RS12695 (JD965_12695) rsfS 2505305..2505661 (-) 357 WP_016938891.1 ribosome silencing factor -
  JD965_RS12700 (JD965_12700) yqeK 2505678..2506238 (-) 561 WP_198697908.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  JD965_RS12705 (JD965_12705) - 2506228..2506797 (-) 570 WP_045926759.1 nicotinate-nucleotide adenylyltransferase -
  JD965_RS12710 (JD965_12710) yhbY 2506808..2507098 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 87364.22 Da        Isoelectric Point: 9.1156

>NTDB_id=518517 JD965_RS12670 WP_198697904.1 2500021..2502372(-) (comEC) [Bacillus siamensis strain B28]
MKYKYLLLPLAAVSATAGIAAANFFWVLFFFLLYLLFIFVKTKQYTPFIVSLISFCLYFFLYTVYDAANVSRYQAGNYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKSLIEQLEPGMHCVFTGSLEQPRHATSPGGFDYKEYLNSQHI
HWLFSVTSIQQCEKSGMPLYKLLNIRKNLISIIQNHVPESSAGIVEALTLGERFSIEDDILNAYQNLGVVHLMAISGMHV
GLITAGIFYVLIRIGLTREKAGVLLLFFLPVYTLLSGAAPSVLRASLMLGFYIAGTLFKRRIHSSAALSVSYLLLLMFNP
YLLWQAGFQLSFAVSAALILSSSILKKAGKRRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPF
SVIGFLLLLLSRQVGECLFYMFDLVMKPVHDFMTYAASVDLFTMIVSKPDFLSLLLLAVSMFTLFAALEKGGFLKLRKSP
LFFCAVLVYLMFRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVVAYPEESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKKHRVKRLIVPVGFVKEPKDQDILKMAKENNIPVVEAKRGDTITAGNLQFQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETDVLKAYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGE
DNRYHHPHEEVLDRLKAYSVNVLRTDLSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=518517 JD965_RS12670 WP_198697904.1 2500021..2502372(-) (comEC) [Bacillus siamensis strain B28]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACCGCGGGAATTGCCGCCGCAAATTTCTTCTGGGT
TCTATTCTTTTTCCTTCTGTATCTTCTCTTTATTTTTGTAAAAACAAAGCAGTACACACCGTTTATCGTCAGCCTCATTT
CTTTTTGTCTTTATTTCTTTCTGTATACGGTTTATGACGCGGCGAATGTATCCCGGTATCAGGCCGGGAACTATACTGAA
CAGGCCGTCATCACAAATATTCCGAAGGTTGACGGGGCGAAAATGTCAGCCGTTATCCGAACTCATGACAAAGAAAAATG
GGCCGCTTCATACAAAATCCGGTCTCTTGAAGAAAAAAGTCTCATTGAACAGCTGGAGCCGGGGATGCACTGCGTGTTTA
CCGGCTCTTTGGAACAGCCCAGACATGCGACGTCTCCCGGAGGTTTTGATTATAAGGAATATCTTAATTCTCAGCATATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCGGCATGCCGCTGTATAAACTGCTGAACATCAGAAA
GAATTTGATTTCGATCATTCAAAATCACGTGCCTGAATCCTCTGCCGGAATTGTCGAAGCGCTGACCTTGGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAACGCTTATCAAAATCTGGGAGTCGTTCATTTAATGGCCATTTCCGGAATGCATGTC
GGTCTGATTACCGCAGGAATATTTTATGTTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGGTTTTACTGCTGTT
TTTTTTACCGGTGTATACGCTGTTAAGCGGTGCGGCCCCGTCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTTTTAAACGCAGAATTCATTCCTCGGCTGCATTGTCCGTATCTTATCTGCTGCTCCTCATGTTTAATCCT
TACCTCCTTTGGCAGGCGGGCTTCCAGCTTTCTTTTGCGGTAAGTGCCGCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGAAGACTTGCCGGGCTTGCGATGGCCTCATTCATTGCGGAGCTCAGTTCGCTTCCGTTTCTGCTCTATC
ATTTTCAGCAGATTTCGCTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCTTTTTATACGTTATTTGTCATTCCGTTT
TCGGTCATCGGTTTCCTTCTTCTCTTACTTTCAAGGCAGGTTGGGGAATGTTTGTTTTATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATGACATATGCGGCATCCGTTGATTTATTTACAATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TCCTTCTGCTTGCGGTTTCCATGTTTACGCTTTTTGCAGCTTTGGAAAAGGGAGGTTTTTTGAAACTCAGGAAATCGCCT
CTTTTTTTCTGCGCGGTTTTGGTTTATTTAATGTTCCGTCCTTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGTGCGCCGCACCGCAAAGGAACCGTAATGGTTGATACAGGGGGAGTGGTGGCTT
ATCCGGAAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGTGAGAAGGTTTTGATTCCGTTTTTGAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCATATCGGGGAAGCCGGAGTATTAATCAAAAA
ACATAGAGTCAAACGGTTGATTGTTCCCGTGGGGTTCGTGAAGGAACCGAAGGATCAGGACATATTGAAGATGGCGAAAG
AAAACAACATTCCCGTTGTCGAAGCAAAGCGGGGAGACACCATTACAGCCGGTAATCTTCAATTTCAGGTGCTGTCCCCG
GAGTCGTCTGACGGAAAGAGTAAGAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGATGTGCTGAAAGCGTATCCGAATCTGAAGGCTGATATATTGAAGGCCGGTC
ATCACGGCAGCAAAAGCTCTACGAGTGAAGCTTTTTTGAAACAGCTTCAGCCGGAAGCGGCACTGATTTCGGCAGGTGAA
GATAATCGTTACCATCATCCGCATGAAGAGGTGCTGGATCGTTTGAAAGCGTACTCTGTCAATGTACTTCGCACCGATCT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCTGTCTTTCCTCCATATGATATAGAAGAAACCA
GGGCGCAGGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-469)

Domain of unknown function (DUF4131) (DUF4131)

(25-171)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

55.829

98.595

0.55