Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JD965_RS12245 Genome accession   NZ_CP066219
Coordinates   2423935..2425005 (-) Length   356 a.a.
NCBI ID   WP_045926720.1    Uniprot ID   A0AAI8HN13
Organism   Bacillus siamensis strain B28     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418935..2430005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JD965_RS12195 (JD965_12195) sipW 2418995..2419579 (-) 585 WP_016938975.1 signal peptidase I SipW -
  JD965_RS12200 (JD965_12200) tapA 2419551..2420222 (-) 672 WP_198697876.1 amyloid fiber anchoring/assembly protein TapA -
  JD965_RS12205 (JD965_12205) - 2420481..2420810 (+) 330 WP_198697877.1 DUF3889 domain-containing protein -
  JD965_RS12210 (JD965_12210) - 2420850..2421029 (-) 180 WP_016938971.1 YqzE family protein -
  JD965_RS12215 (JD965_12215) comGG 2421086..2421463 (-) 378 WP_188154558.1 competence type IV pilus minor pilin ComGG Machinery gene
  JD965_RS12220 (JD965_12220) comGF 2421464..2421961 (-) 498 WP_232904794.1 competence type IV pilus minor pilin ComGF -
  JD965_RS12225 (JD965_12225) comGE 2421873..2422187 (-) 315 WP_045926718.1 competence type IV pilus minor pilin ComGE Machinery gene
  JD965_RS12230 (JD965_12230) comGD 2422171..2422608 (-) 438 WP_045926719.1 competence type IV pilus minor pilin ComGD Machinery gene
  JD965_RS12235 (JD965_12235) comGC 2422598..2422906 (-) 309 WP_016938966.1 competence type IV pilus major pilin ComGC Machinery gene
  JD965_RS12240 (JD965_12240) comGB 2422911..2423948 (-) 1038 WP_198697878.1 competence type IV pilus assembly protein ComGB Machinery gene
  JD965_RS12245 (JD965_12245) comGA 2423935..2425005 (-) 1071 WP_045926720.1 competence type IV pilus ATPase ComGA Machinery gene
  JD965_RS12250 (JD965_12250) - 2425198..2426148 (-) 951 WP_198697879.1 magnesium transporter CorA family protein -
  JD965_RS12255 (JD965_12255) - 2426296..2427597 (+) 1302 WP_198697880.1 hemolysin family protein -
  JD965_RS12260 (JD965_12260) - 2427635..2428465 (-) 831 WP_198697881.1 STAS domain-containing protein -
  JD965_RS12270 (JD965_12270) - 2428675..2429055 (-) 381 WP_045926723.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JD965_RS12275 (JD965_12275) - 2429292..2429537 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40409.64 Da        Isoelectric Point: 9.2036

>NTDB_id=518515 JD965_RS12245 WP_045926720.1 2423935..2425005(-) (comGA) [Bacillus siamensis strain B28]
MENIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPSETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFPGAGAALLSFLKHSHGLILFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKAENCSVYCRLYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=518515 JD965_RS12245 WP_045926720.1 2423935..2425005(-) (comGA) [Bacillus siamensis strain B28]
ATGGAGAATATAGAAACATACAGCAGGAACATTATAAACGAGGCCTATACGGCAAGAGCTTCAGATATACACATCGTGCC
GCGTGAAAGGGATGCTTTCATTCATTTTCGCATTGGTCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCAGCGATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGATCACTGTCT
CTGCCGCTTCCATCTGAAACCGTTCATTTAAGGATGTCAACCTTACCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCCGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCCCACGGCCTGATTCTTTTTACCGGTCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAATTAT
GCAAAACGTCATTTCAACCGGCGGATTGTCACGCTTGAAGATCCCGTAGAAACGAGAGATGAAGATGTTTTACAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCTATGACGGGGCATCTTGTTCTTTCAAGCCTACAT
ACAAGAGATGCAAAAGGCGCTATATACAGGCTGCTGGAGTTTGGGGTGAACATGACGGAAATTGAACAAACCGTTATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTTTGCAAAGCGGAAAACTGTTCCGTGTACTGCCGGCTGTACA
GGCAGACGCGCCGTGCGGGAATCTATGAACTACTTTACGGGAAAAATCTCACCCAATGCTTTCAGGAAGCAAAAGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCCGAAAAGGAATCGCGCTCGGCTATGTCACGACTGACAACTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379