Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   JD965_RS12240 Genome accession   NZ_CP066219
Coordinates   2422911..2423948 (-) Length   345 a.a.
NCBI ID   WP_198697878.1    Uniprot ID   -
Organism   Bacillus siamensis strain B28     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2417911..2428948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JD965_RS12190 (JD965_12190) tasA 2418146..2418931 (-) 786 WP_047475632.1 biofilm matrix protein TasA -
  JD965_RS12195 (JD965_12195) sipW 2418995..2419579 (-) 585 WP_016938975.1 signal peptidase I SipW -
  JD965_RS12200 (JD965_12200) tapA 2419551..2420222 (-) 672 WP_198697876.1 amyloid fiber anchoring/assembly protein TapA -
  JD965_RS12205 (JD965_12205) - 2420481..2420810 (+) 330 WP_198697877.1 DUF3889 domain-containing protein -
  JD965_RS12210 (JD965_12210) - 2420850..2421029 (-) 180 WP_016938971.1 YqzE family protein -
  JD965_RS12215 (JD965_12215) comGG 2421086..2421463 (-) 378 WP_188154558.1 competence type IV pilus minor pilin ComGG Machinery gene
  JD965_RS12220 (JD965_12220) comGF 2421464..2421961 (-) 498 WP_232904794.1 competence type IV pilus minor pilin ComGF -
  JD965_RS12225 (JD965_12225) comGE 2421873..2422187 (-) 315 WP_045926718.1 competence type IV pilus minor pilin ComGE Machinery gene
  JD965_RS12230 (JD965_12230) comGD 2422171..2422608 (-) 438 WP_045926719.1 competence type IV pilus minor pilin ComGD Machinery gene
  JD965_RS12235 (JD965_12235) comGC 2422598..2422906 (-) 309 WP_016938966.1 competence type IV pilus major pilin ComGC Machinery gene
  JD965_RS12240 (JD965_12240) comGB 2422911..2423948 (-) 1038 WP_198697878.1 competence type IV pilus assembly protein ComGB Machinery gene
  JD965_RS12245 (JD965_12245) comGA 2423935..2425005 (-) 1071 WP_045926720.1 competence type IV pilus ATPase ComGA Machinery gene
  JD965_RS12250 (JD965_12250) - 2425198..2426148 (-) 951 WP_198697879.1 magnesium transporter CorA family protein -
  JD965_RS12255 (JD965_12255) - 2426296..2427597 (+) 1302 WP_198697880.1 hemolysin family protein -
  JD965_RS12260 (JD965_12260) - 2427635..2428465 (-) 831 WP_198697881.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39630.03 Da        Isoelectric Point: 9.4238

>NTDB_id=518514 JD965_RS12240 WP_198697878.1 2422911..2423948(-) (comGB) [Bacillus siamensis strain B28]
MKRIKRTWPLKDQAAFLKRLGEMIEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYEVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQVKKMLRYPMFLICSVCVMFYILQSMIIPQFSGIYQSMNMNTSGCVSFIFSFF
QHFHEACALVLSAALSLFLFVWFLCKKKSPQDKMLIVVKIPLIGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEADMLITRLKAGETIESALSGHPCYEKGLAAAVSHGQANGRLHRELYTYSQFMMERLEQNAEKYTGILQPVIY
GVVAGMILIIYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=518514 JD965_RS12240 WP_198697878.1 2422911..2423948(-) (comGB) [Bacillus siamensis strain B28]
ATGAAGCGGATTAAAAGAACCTGGCCGTTGAAGGATCAAGCTGCTTTTTTGAAAAGGCTCGGTGAAATGATCGAAAAAGG
GTACAGTCTGATCGAAGGGCTAAGACTGCTGAAACTCCAGCTTCATAAACGCCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGCGATGCGTTTTACGAAGTGCTTGAGGCATTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAAAAACACGGAGAATTGCCCGGGGCGATGAAACAAAGCGGCGAATTGTTACAGAGAAAGCTCATGCAGACGAA
TCAAGTCAAAAAAATGCTCCGGTATCCGATGTTTTTAATCTGCTCCGTCTGTGTCATGTTTTATATTCTGCAAAGTATGA
TCATCCCGCAATTTTCAGGGATTTATCAATCAATGAATATGAATACTTCCGGTTGCGTCAGTTTCATTTTTTCATTTTTT
CAGCATTTTCACGAAGCTTGCGCACTGGTGCTGTCAGCCGCTCTCAGCTTGTTTTTGTTCGTTTGGTTTTTATGTAAGAA
AAAATCCCCGCAAGACAAAATGCTTATTGTTGTGAAAATCCCCTTAATAGGAAAAGCAGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGTCTTTCTATCTATGACAGCTTAACGGCTTTTAAGGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCGGATATGCTCATTACACGTTTGAAAGCAGGTGAGACAATAGAATCCGCTCTTTC
TGGGCATCCGTGTTATGAAAAAGGTCTAGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCAGGCTTCATCGCGAGCTTT
ATACGTACAGTCAGTTTATGATGGAGCGCCTTGAACAAAACGCGGAGAAGTATACAGGCATTCTCCAGCCTGTCATTTAC
GGCGTTGTGGCCGGTATGATTCTGATTATCTATATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

64.087

93.623

0.6