Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I6I28_RS00340 Genome accession   NZ_CP066062
Coordinates   63926..64900 (-) Length   324 a.a.
NCBI ID   WP_049405253.1    Uniprot ID   A0A1E9ACU3
Organism   Staphylococcus pettenkoferi strain FDAARGOS_1071     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58926..69900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I28_RS00305 (I6I28_00305) gcvT 59487..60578 (-) 1092 WP_002472490.1 glycine cleavage system aminomethyltransferase GcvT -
  I6I28_RS00310 (I6I28_00310) - 60757..61230 (-) 474 WP_049405250.1 shikimate kinase -
  I6I28_RS00315 (I6I28_00315) - 61437..61826 (-) 390 WP_169931256.1 ComGF family competence protein -
  I6I28_RS00320 (I6I28_00320) - 61852..62139 (-) 288 WP_088606290.1 hypothetical protein -
  I6I28_RS00325 (I6I28_00325) comGD 62126..62578 (-) 453 WP_198465111.1 competence type IV pilus minor pilin ComGD -
  I6I28_RS00330 (I6I28_00330) comGC 62556..62873 (-) 318 WP_002472491.1 competence type IV pilus major pilin ComGC Machinery gene
  I6I28_RS00335 (I6I28_00335) comGB 62887..63954 (-) 1068 WP_049405252.1 competence type IV pilus assembly protein ComGB -
  I6I28_RS00340 (I6I28_00340) comGA 63926..64900 (-) 975 WP_049405253.1 competence type IV pilus ATPase ComGA Machinery gene
  I6I28_RS00345 (I6I28_00345) - 64967..65593 (-) 627 WP_049405255.1 MBL fold metallo-hydrolase -
  I6I28_RS00350 (I6I28_00350) - 65593..65919 (-) 327 WP_002472477.1 MTH1187 family thiamine-binding protein -
  I6I28_RS00355 (I6I28_00355) - 65919..66905 (-) 987 WP_049405257.1 ROK family glucokinase -
  I6I28_RS00360 (I6I28_00360) - 66910..67104 (-) 195 WP_049405258.1 YqgQ family protein -
  I6I28_RS00365 (I6I28_00365) - 67106..68545 (-) 1440 WP_103370190.1 rhomboid family intramembrane serine protease -
  I6I28_RS00370 (I6I28_00370) - 68558..69094 (-) 537 WP_103370191.1 5-formyltetrahydrofolate cyclo-ligase -
  I6I28_RS00375 (I6I28_00375) rpmG 69556..69705 (-) 150 WP_002472489.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 37285.28 Da        Isoelectric Point: 6.5849

>NTDB_id=517004 I6I28_RS00340 WP_049405253.1 63926..64900(-) (comGA) [Staphylococcus pettenkoferi strain FDAARGOS_1071]
MKLLFKEMISRAIKHEASDIHFIPSEEEVFVKFRINDNLQSFESIQHETYTKLLTYMKYLAGLDVSTHIIAQSGRYLFKA
KRPYYLRISTLPLSLGIESCVIRLIPQYFEQHSETKHGDILYKLMNKKQGLILFSGPTGSGKSTLMYQMINYAKDQLNLN
IITVEDPIEQKINGITQISINEKAGINYASSFKAILRCDPDIILIGEIRDANVAKYVIQASMSGHLVLSTIHANDCRGTL
LRLLEMGISEQEIKQALINITNQRLIPISDDKRELVCEMMMKEDIAYFFEHEQTMPYTFQNLSYILKEMSKEGVICEDTV
DRYL

Nucleotide


Download         Length: 975 bp        

>NTDB_id=517004 I6I28_RS00340 WP_049405253.1 63926..64900(-) (comGA) [Staphylococcus pettenkoferi strain FDAARGOS_1071]
TTGAAACTATTATTCAAAGAAATGATTTCAAGGGCAATTAAGCACGAAGCGTCGGATATTCACTTTATCCCATCAGAAGA
GGAAGTATTTGTGAAATTTCGTATCAATGATAACCTGCAGAGTTTTGAATCAATTCAACACGAAACTTATACGAAATTAC
TAACGTATATGAAGTATTTGGCAGGCCTCGATGTGTCAACCCATATCATCGCACAGAGCGGTCGCTATTTATTCAAAGCT
AAGCGACCTTATTATTTACGTATTTCGACATTGCCACTGTCCTTAGGGATTGAAAGTTGTGTTATTCGTTTAATACCGCA
ATACTTTGAACAGCATTCTGAAACGAAACACGGCGACATTTTGTATAAGTTGATGAATAAGAAACAAGGTTTGATTCTCT
TTAGTGGGCCAACAGGATCAGGTAAAAGCACTTTAATGTATCAGATGATTAATTACGCTAAAGATCAATTAAATTTGAAT
ATCATCACTGTGGAAGATCCGATTGAACAAAAAATAAATGGCATTACTCAAATCTCGATTAACGAAAAGGCGGGGATTAA
CTATGCGAGTTCCTTTAAAGCGATACTACGCTGTGATCCAGATATCATACTTATCGGTGAAATTCGTGATGCTAATGTAG
CGAAATATGTGATTCAAGCAAGTATGAGCGGGCACCTTGTCCTTTCAACAATTCACGCGAATGATTGTAGAGGTACTTTA
CTTCGTCTGTTGGAAATGGGGATATCTGAACAAGAAATTAAGCAAGCGTTGATTAATATTACGAATCAACGTCTCATTCC
TATCAGCGATGATAAGCGAGAATTGGTCTGTGAAATGATGATGAAAGAGGATATCGCTTACTTTTTCGAGCACGAGCAAA
CGATGCCTTATACTTTCCAGAATTTATCTTACATTCTTAAAGAAATGTCGAAAGAAGGTGTTATTTGTGAAGACACCGTC
GATCGATATCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E9ACU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

61.42

100

0.614
  comGA Staphylococcus aureus N315

61.42

100

0.614
  comGA Bacillus subtilis subsp. subtilis str. 168

34.302

100

0.364