Detailed information NTG0051664

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: THIVI_RS23710
• Genome accession: NC_018012
• Coordinates: 2730940..2731434 (+)
• Length: 164 a.a.
• NCBI ID: WP_014778869.1
• Uniprot ID: I3YBQ7
• Organism: Thiocystis violascens DSM 198
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2723432..2734595	2730940..2731434	within	0

Gene organization within MGE regions

Location: 2723432..2734595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THIVI_RS12005 (Thivi_2477)	-	2723432..2724313 (+)	882	WP_014778861.1	hypothetical protein	-
THIVI_RS23705	-	2724334..2724702 (+)	369	Protein_2457	ATP-binding cassette domain-containing protein	-
THIVI_RS12015 (Thivi_2479)	-	2724851..2725288 (+)	438	WP_014778863.1	DUF2721 domain-containing protein	-
THIVI_RS12020 (Thivi_2480)	-	2725332..2726411 (-)	1080	WP_014778864.1	glycosyltransferase	-
THIVI_RS12025 (Thivi_2481)	-	2726453..2727541 (-)	1089	WP_014778865.1	glycosyltransferase family 4 protein	-
THIVI_RS12030 (Thivi_2482)	-	2727538..2728572 (-)	1035	WP_014778866.1	glycosyltransferase family 9 protein	-
THIVI_RS12035 (Thivi_2483)	-	2728694..2730067 (+)	1374	WP_245537263.1	O-antigen ligase family protein	-
THIVI_RS12040 (Thivi_2484)	-	2730059..2730535 (-)	477	WP_014778868.1	SEL1-like repeat protein	-
THIVI_RS23710 (Thivi_2485)	pilA	2730940..2731434 (+)	495	WP_014778869.1	pilin	Machinery gene
THIVI_RS12050 (Thivi_2486)	-	2731467..2732450 (-)	984	WP_014778870.1	IS110 family transposase	-
THIVI_RS12055 (Thivi_2487)	-	2733256..2733525 (+)	270	WP_014778871.1	DUF4160 domain-containing protein	-
THIVI_RS12060 (Thivi_2488)	-	2733536..2733775 (+)	240	WP_014778872.1	DUF2442 domain-containing protein	-
THIVI_RS12065	-	2734368..2734595 (-)	228	WP_052315021.1	hypothetical protein	-

Sequence

Protein

• Length: 164 a.a.
• Molecular weight: 16172.53 Da
• Isoelectric Point: 8.4687

>NTDB_id=51664 THIVI_RS23710 WP_014778869.1 2730940..2731434(+) (pilA) [Thiocystis violascens DSM 198]
MKKYQQGFTLIELMIVVAIIGILAAIALPAYQDYMVRARVTEGLTAASAAKVNVVDVLASGNPSAAAGYGNGYTSPTATE
NVTSVAIAAGTGVITVTMTAAAGNGTLTLTPNAPSGTALPVGTAAFTPPGDSVAWRCGAAGATSTFAGFTAGTLPARFAP
SACK

Nucleotide

• Length: 495 bp

>NTDB_id=51664 THIVI_RS23710 WP_014778869.1 2730940..2731434(+) (pilA) [Thiocystis violascens DSM 198]
ATGAAAAAGTATCAACAAGGCTTTACTTTGATCGAACTGATGATCGTGGTGGCGATTATTGGGATTTTGGCGGCGATTGC
GTTGCCAGCGTATCAGGATTATATGGTCAGGGCTCGCGTCACCGAAGGTTTGACGGCTGCATCTGCTGCAAAGGTAAATG
TCGTCGATGTGCTAGCTTCCGGTAATCCAAGTGCTGCTGCTGGCTATGGAAATGGTTACACAAGCCCAACGGCAACGGAA
AATGTCACCAGCGTTGCGATCGCTGCTGGTACGGGCGTTATTACCGTGACCATGACCGCTGCGGCTGGCAACGGAACCCT
GACACTGACACCGAATGCACCATCTGGCACCGCCCTTCCTGTTGGTACAGCCGCCTTTACTCCTCCGGGTGATTCCGTAG
CGTGGCGCTGCGGTGCTGCCGGTGCTACTTCTACTTTTGCTGGCTTCACTGCCGGTACGCTTCCAGCACGCTTTGCGCCT
TCCGCGTGTAAATAG

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (2-28)

• Pilin (bacterial filament) (Pilin) (36-164)

3D structure

• AlphaFold DB: I3YBQ7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Ralstonia pseudosolanacearum GMI1000	50.588	100	0.524
pilA2	Legionella pneumophila strain ERS1305867	50.625	97.561	0.494
pilA2	Legionella pneumophila str. Paris	50.625	97.561	0.494
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	37.949	100	0.451
comP	Acinetobacter baylyi ADP1	41.52	100	0.433