Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I6I17_RS05105 Genome accession   NZ_CP065999
Coordinates   1018296..1020413 (+) Length   705 a.a.
NCBI ID   WP_003780878.1    Uniprot ID   F0EW58
Organism   Kingella denitrificans strain FDAARGOS_1060     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1013296..1025413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I17_RS05085 (I6I17_05085) pilM 1015323..1016432 (+) 1110 WP_003780872.1 type IV pilus assembly protein PilM -
  I6I17_RS05090 (I6I17_05090) - 1016434..1017069 (+) 636 WP_003780873.1 PilN domain-containing protein -
  I6I17_RS05095 (I6I17_05095) - 1017069..1017677 (+) 609 WP_003780875.1 type 4a pilus biogenesis protein PilO -
  I6I17_RS05100 (I6I17_05100) - 1017711..1018259 (+) 549 WP_040564118.1 pilus assembly protein PilP -
  I6I17_RS05105 (I6I17_05105) pilQ 1018296..1020413 (+) 2118 WP_003780878.1 type IV pilus secretin PilQ Machinery gene
  I6I17_RS05110 (I6I17_05110) - 1020469..1020981 (+) 513 WP_232618030.1 shikimate kinase -
  I6I17_RS05115 (I6I17_05115) aroB 1020978..1022060 (+) 1083 WP_003780884.1 3-dehydroquinate synthase -
  I6I17_RS05120 (I6I17_05120) - 1022081..1022506 (+) 426 WP_003780886.1 ProQ/FINO family protein -
  I6I17_RS05125 (I6I17_05125) kdsA 1022600..1023445 (+) 846 WP_040564527.1 3-deoxy-8-phosphooctulonate synthase -
  I6I17_RS05130 (I6I17_05130) - 1023623..1024615 (+) 993 WP_003780890.1 tRNA-dihydrouridine synthase -
  I6I17_RS05135 (I6I17_05135) deoD 1024628..1025341 (+) 714 WP_003780891.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 76758.87 Da        Isoelectric Point: 9.9881

>NTDB_id=516249 I6I17_RS05105 WP_003780878.1 1018296..1020413(+) (pilQ) [Kingella denitrificans strain FDAARGOS_1060]
MKTNSLKKLSVFGLGLMMQAAFAGNITDVNVSVLPDQQRVIKLKFDRDPVEPSGFITSSPARIALDFRNTGVKLAQSALT
FNDALLNQVVAAQNDANTRVLLGLTKDAQYNAQVRGNEVWIYVSESASTNTAATNAGVVAGARNTYVPTANASGFNIDFR
KASQNAGVVEFSSAHGGQPQIKAQNDRIVVTLKNYALPTQEQRNLDVTDFSTPVKAITVKRLGNDTQIVIRNQGTWQHKV
TENNGRYAIQVSPKRNVAEASVLSGRPKAQNFTGKRISLDFQNVDVRTVLQVLAKESGMNIVASDSVQGKMTLSLKDVPW
DQALDLVLDARNLAMRKHGNIINIAPRQELREKDLEEAKGRQEMEQLGPLLSQSFQLKYKNVEEFKNILRLSDNGSTNGD
NSKTILSPRGSALIDPATNTLIITDIQTVIQKFQRLIEELDVPAKQVMVEARIVEAADGVARDLGVKFGANRQGSTSWGS
NWGNGVEANRRYVGNGTGSGSNGGVNFAPNVNLPVSSATSSIAVIRSWASGALGLELSAMETDNLTKTISTPRVLTQDRK
EAEIRQGTQIPYTTRGSNGEVTTSFKDAILSLKVTPRITPDSKIILDITINKDEPDYTRVNVEGEPGITTRAVKTQAMIE
DGGTLVVGGIYQEQMSNNISKVPFLGDIPVLGNLFKSRSRSHTRNELLFFITPRIMGGESTVLRY

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=516249 I6I17_RS05105 WP_003780878.1 1018296..1020413(+) (pilQ) [Kingella denitrificans strain FDAARGOS_1060]
ATGAAAACAAATTCACTGAAAAAGTTATCAGTATTTGGTTTGGGTTTGATGATGCAGGCTGCTTTTGCTGGAAATATTAC
CGATGTGAATGTATCGGTATTACCAGATCAACAGCGTGTGATCAAATTAAAATTTGACAGAGATCCAGTAGAACCAAGCG
GTTTTATTACCAGCTCGCCTGCACGCATTGCTTTGGATTTTCGTAATACTGGTGTGAAACTGGCTCAATCAGCATTAACG
TTTAATGATGCATTATTGAACCAAGTTGTGGCAGCACAAAATGATGCTAATACACGGGTTCTGTTGGGTTTGACAAAAGA
TGCACAATATAATGCCCAAGTTCGTGGCAATGAAGTATGGATTTATGTAAGTGAATCTGCATCAACCAATACAGCAGCAA
CCAATGCGGGTGTTGTGGCAGGGGCTCGTAACACCTATGTGCCCACTGCGAATGCGAGTGGTTTCAACATTGATTTCCGT
AAAGCATCACAAAATGCAGGTGTTGTCGAGTTTAGTTCTGCTCATGGTGGCCAACCACAAATCAAGGCGCAGAATGACCG
CATTGTAGTTACTTTGAAAAACTATGCTTTGCCTACGCAAGAGCAGCGCAATTTGGATGTAACAGATTTTTCAACGCCAG
TAAAAGCGATTACAGTGAAACGCTTGGGTAACGATACGCAGATTGTTATCCGCAACCAAGGCACTTGGCAGCATAAAGTG
ACTGAAAATAATGGTCGTTATGCTATCCAAGTTTCGCCTAAACGTAATGTTGCGGAAGCTTCAGTACTTTCAGGTCGCCC
GAAAGCACAAAATTTTACGGGTAAACGAATTTCTTTGGATTTCCAAAACGTTGATGTACGTACTGTATTGCAAGTATTGG
CCAAAGAATCTGGCATGAATATTGTTGCCAGTGATTCTGTGCAAGGTAAAATGACCTTGTCATTGAAAGATGTGCCTTGG
GATCAGGCGTTGGATTTAGTTTTGGATGCGCGTAATTTGGCTATGCGCAAACACGGCAATATTATTAACATTGCCCCACG
TCAAGAATTGAGAGAAAAAGACCTTGAAGAGGCGAAGGGTCGTCAAGAAATGGAACAGTTGGGACCTCTTTTGTCTCAAA
GCTTCCAGTTGAAATATAAAAACGTAGAAGAATTTAAAAATATTTTGCGGTTGTCCGATAATGGTTCAACAAATGGTGAT
AATAGTAAGACTATTTTGAGTCCTCGCGGTAGTGCATTAATTGACCCTGCAACCAATACATTAATTATTACTGACATACA
AACTGTTATTCAAAAATTCCAGCGTTTAATTGAAGAGTTGGATGTGCCAGCCAAACAAGTGATGGTGGAAGCACGTATTG
TTGAAGCGGCAGACGGTGTAGCTCGTGATTTGGGTGTGAAATTTGGCGCTAACCGTCAAGGTTCTACTTCATGGGGTAGT
AACTGGGGTAATGGTGTTGAAGCAAATCGCCGTTATGTGGGCAATGGTACAGGGTCTGGCTCGAATGGTGGTGTGAACTT
TGCACCTAACGTGAATCTGCCAGTAAGCAGCGCCACAAGCTCTATTGCAGTTATCCGTTCTTGGGCTTCAGGCGCGTTGG
GCTTGGAATTGTCTGCAATGGAAACAGATAACTTGACGAAAACAATTTCTACGCCACGTGTATTGACACAAGACCGTAAA
GAAGCCGAAATTCGCCAAGGTACTCAAATTCCTTATACCACGCGCGGTAGTAATGGTGAGGTAACAACATCATTTAAAGA
CGCTATTTTGAGCTTGAAAGTAACGCCACGTATTACGCCAGATTCCAAAATTATCTTGGATATTACGATTAACAAAGACG
AGCCCGACTATACTCGTGTAAACGTTGAAGGCGAACCTGGTATTACAACGCGCGCCGTGAAAACTCAGGCGATGATTGAA
GATGGTGGCACTTTGGTTGTGGGTGGTATCTATCAAGAACAAATGTCTAATAACATTAGTAAAGTACCGTTCTTGGGCGA
TATTCCTGTTTTGGGTAATTTGTTCAAATCACGCTCACGCAGCCACACGCGTAATGAACTGTTGTTCTTCATCACACCAC
GTATTATGGGTGGTGAGAGCACTGTGTTGCGCTACTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(36-115)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-446)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II and III secretion system protein (Secretin)

(539-696)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F0EW58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

50.826

100

0.523
  pilQ Neisseria meningitidis MC58

55

79.433

0.437
  pilQ Neisseria meningitidis M1080

54.643

79.433

0.434