Detailed information NTG0515836

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KZH42_RS01020
• Genome accession: NZ_CP079941
• Coordinates: 195405..196631 (-)
• Length: 408 a.a.
• NCBI ID: WP_002218483.1
• Uniprot ID: Q9K1N4
• Organism: Neisseria meningitidis strain C311
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 190405..201631

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZH42_RS01015 (KZH42_01015)	yccS	192969..195119 (-)	2151	WP_002221798.1	YccS family putative transporter	-
KZH42_RS01020 (KZH42_01020)	pilU	195405..196631 (-)	1227	WP_002218483.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KZH42_RS01025 (KZH42_01025)	pilT	196794..197837 (-)	1044	WP_002218487.1	type IV pilus twitching motility protein PilT	Machinery gene
KZH42_RS01030 (KZH42_01030)	-	197957..198643 (+)	687	WP_002243931.1	YggS family pyridoxal phosphate-dependent enzyme	-
KZH42_RS01035 (KZH42_01035)	-	198643..199050 (+)	408	WP_002243932.1	hypothetical protein	-
KZH42_RS01040 (KZH42_01040)	proC	199081..199890 (+)	810	WP_225905354.1	pyrroline-5-carboxylate reductase	-
KZH42_RS01045 (KZH42_01045)	dksA	200037..200453 (+)	417	WP_002215267.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45674.28 Da
• Isoelectric Point: 6.9640

>NTDB_id=515836 KZH42_RS01020 WP_002218483.1 195405..196631(-) (pilU) [Neisseria meningitidis strain C311]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=515836 KZH42_RS01020 WP_002218483.1 195405..196631(-) (pilU) [Neisseria meningitidis strain C311]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAATTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q9K1N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.598	88.48	0.395
pilU	Pseudomonas stutzeri DSM 10701	44.663	87.255	0.39
pilU	Vibrio cholerae strain A1552	46.429	82.353	0.382