Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KZH40_RS04335 Genome accession   NZ_CP079921
Coordinates   975056..975502 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   Q8VU03
Organism   Actinobacillus pleuropneumoniae strain P1875     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 970056..980502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH40_RS04310 (KZH40_04350) yacG 970963..971151 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  KZH40_RS04315 (KZH40_04355) coaE 971133..971771 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  KZH40_RS04320 (KZH40_04360) - 971793..972431 (-) 639 WP_005604550.1 prepilin peptidase -
  KZH40_RS04325 (KZH40_04365) pilC 972458..973660 (-) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  KZH40_RS04330 (KZH40_04370) pilB 973644..975038 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  KZH40_RS04335 (KZH40_04375) pilA 975056..975502 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  KZH40_RS04340 (KZH40_04380) radA/sms 975684..977063 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  KZH40_RS04345 (KZH40_04385) - 977107..977340 (-) 234 WP_005597399.1 YgjV family protein -
  KZH40_RS04350 (KZH40_04390) folA 977467..977955 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  KZH40_RS04355 (KZH40_04395) proB 978042..979133 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  KZH40_RS04360 (KZH40_04400) - 979146..979922 (+) 777 WP_005601215.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=515537 KZH40_RS04335 WP_005597397.1 975056..975502(-) (pilA) [Actinobacillus pleuropneumoniae strain P1875]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=515537 KZH40_RS04335 WP_005597397.1 975056..975502(-) (pilA) [Actinobacillus pleuropneumoniae strain P1875]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VU03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527