Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   I8N73_RS12675 Genome accession   NZ_CP065791
Coordinates   2625173..2625973 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain LBUM279     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2620173..2630973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8N73_RS12645 (I8N73_12645) - 2620718..2621350 (+) 633 WP_007408365.1 helix-turn-helix domain-containing protein -
  I8N73_RS12650 (I8N73_12650) - 2621641..2621778 (+) 138 WP_014470646.1 hypothetical protein -
  I8N73_RS12655 (I8N73_12655) - 2621872..2622990 (+) 1119 WP_199022654.1 NADH-dependent flavin oxidoreductase -
  I8N73_RS12660 (I8N73_12660) - 2623113..2623328 (+) 216 WP_144663997.1 spore germination protein -
  I8N73_RS12665 (I8N73_12665) - 2623454..2623666 (+) 213 WP_003153181.1 spore germination protein -
  I8N73_RS12670 (I8N73_12670) alr 2623843..2625024 (+) 1182 WP_007408361.1 alanine racemase -
  I8N73_RS12675 (I8N73_12675) spo0A 2625173..2625973 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  I8N73_RS12680 (I8N73_12680) spoIVB 2626254..2627534 (-) 1281 WP_033574141.1 SpoIVB peptidase -
  I8N73_RS12685 (I8N73_12685) recN 2627698..2629428 (-) 1731 WP_053103983.1 DNA repair protein RecN Machinery gene
  I8N73_RS12690 (I8N73_12690) ahrC 2629460..2629909 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  I8N73_RS12695 (I8N73_12695) - 2630006..2630851 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=514547 I8N73_RS12675 WP_003153177.1 2625173..2625973(-) (spo0A) [Bacillus velezensis strain LBUM279]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=514547 I8N73_RS12675 WP_003153177.1 2625173..2625973(-) (spo0A) [Bacillus velezensis strain LBUM279]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCTGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAATTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97