NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	I8N72_RS12155	Genome accession	NZ_CP065790
Coordinates	2520322..2520936 (-)	Length	204 a.a.
NCBI ID	WP_015387983.1	Uniprot ID	A0A6A8LHQ3
Organism	Bacillus velezensis strain LBUM1082
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2515322..2525936

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I8N72_RS12125 (I8N72_12125)	rpsT	2515622..2515888 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
I8N72_RS12130 (I8N72_12130)	holA	2515905..2516946 (-)	1042	Protein_2357	DNA polymerase III subunit delta	-
I8N72_RS12135 (I8N72_12135)	-	2516986..2517140 (-)	155	Protein_2358	hypothetical protein	-
I8N72_RS12140 (I8N72_12140)	-	2517181..2517315 (+)	135	WP_003152870.1	YqzM family protein	-
I8N72_RS12145 (I8N72_12145)	comEC	2517334..2519685 (-)	2352	WP_015387984.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
I8N72_RS12150 (I8N72_12150)	-	2519686..2520255 (-)	570	WP_003152868.1	ComE operon protein 2	-
I8N72_RS12155 (I8N72_12155)	comEA	2520322..2520936 (-)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
I8N72_RS12160 (I8N72_12160)	comER	2520995..2521816 (+)	822	WP_003152866.1	late competence protein ComER	-
I8N72_RS12165 (I8N72_12165)	-	2521885..2522622 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
I8N72_RS12170 (I8N72_12170)	rsfS	2522619..2522975 (-)	357	WP_014305451.1	ribosome silencing factor	-
I8N72_RS12175 (I8N72_12175)	yqeK	2522972..2523553 (-)	582	WP_015387982.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
I8N72_RS12180 (I8N72_12180)	-	2523543..2524112 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
I8N72_RS12185 (I8N72_12185)	yhbY	2524123..2524413 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
I8N72_RS12190 (I8N72_12190)	aroE	2524407..2525249 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22255.41 Da Isoelectric Point: 5.5211

                                >NTDB_id=514486 I8N72_RS12155 WP_015387983.1 2520322..2520936(-) (comEA) [Bacillus velezensis strain LBUM1082]

MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=514486 I8N72_RS12155 WP_015387983.1 2520322..2520936(-) (comEA) [Bacillus velezensis strain LBUM1082]

ATGACGAAAGCAATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0514486