Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KV188_RS01345 Genome accession   NZ_CP078508
Coordinates   290747..291193 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   Q8VU03
Organism   Actinobacillus pleuropneumoniae serovar 8 str. 405     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 285747..296193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KV188_RS01320 (KV188_01320) yacG 286654..286842 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  KV188_RS01325 (KV188_01325) coaE 286824..287462 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  KV188_RS01330 (KV188_01330) - 287484..288122 (-) 639 WP_005604550.1 prepilin peptidase -
  KV188_RS01335 (KV188_01335) pilC 288149..289351 (-) 1203 WP_218633985.1 type II secretion system F family protein Machinery gene
  KV188_RS01340 (KV188_01340) pilB 289335..290729 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  KV188_RS01345 (KV188_01345) pilA 290747..291193 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  KV188_RS01350 (KV188_01350) radA/sms 291375..292754 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  KV188_RS01355 (KV188_01355) - 292798..293031 (-) 234 WP_005597399.1 YgjV family protein -
  KV188_RS01360 (KV188_01360) - 293158..293646 (-) 489 WP_218633986.1 dihydrofolate reductase -
  KV188_RS01365 (KV188_01365) proB 293733..294824 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  KV188_RS01370 (KV188_01370) - 294837..295613 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=514022 KV188_RS01345 WP_005597397.1 290747..291193(-) (pilA) [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=514022 KV188_RS01345 WP_005597397.1 290747..291193(-) (pilA) [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VU03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527