Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   I6G22_RS00775 Genome accession   NZ_CP065737
Coordinates   96601..97950 (+) Length   449 a.a.
NCBI ID   WP_025017173.1    Uniprot ID   A0A0B8R081
Organism   Lactococcus lactis strain FDAARGOS_865     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 91601..102950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G22_RS00750 (I6G22_00750) glyQ 92117..93070 (-) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -
  I6G22_RS00755 (I6G22_00755) - 93356..94102 (-) 747 WP_023189339.1 bifunctional metallophosphatase/5'-nucleotidase -
  I6G22_RS00760 (I6G22_00760) - 94217..95236 (-) 1020 WP_012897709.1 DUF475 domain-containing protein -
  I6G22_RS00765 (I6G22_00765) - 95284..95781 (-) 498 WP_023189340.1 VanZ family protein -
  I6G22_RS00770 (I6G22_00770) - 95915..96544 (-) 630 WP_003130660.1 YigZ family protein -
  I6G22_RS00775 (I6G22_00775) comFA 96601..97950 (+) 1350 WP_025017173.1 DEAD/DEAH box helicase Machinery gene
  I6G22_RS00780 (I6G22_00780) - 98011..98589 (-) 579 WP_023189343.1 nucleotidyltransferase family protein -
  I6G22_RS00785 (I6G22_00785) - 98603..99058 (-) 456 WP_003130653.1 diacylglycerol kinase family protein -
  I6G22_RS00790 (I6G22_00790) ybeY 99042..99530 (-) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  I6G22_RS00795 (I6G22_00795) - 99682..100161 (-) 480 WP_003130650.1 NUDIX hydrolase -
  I6G22_RS00800 (I6G22_00800) - 100219..101187 (-) 969 WP_003130649.1 PhoH family protein -
  I6G22_RS00805 (I6G22_00805) - 101289..102821 (-) 1533 WP_023189345.1 Nramp family divalent metal transporter -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51491.09 Da        Isoelectric Point: 10.1773

>NTDB_id=513703 I6G22_RS00775 WP_025017173.1 96601..97950(+) (comFA) [Lactococcus lactis strain FDAARGOS_865]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPNLGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKNRYHLISRITNSKRSWCS

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=513703 I6G22_RS00775 WP_025017173.1 96601..97950(+) (comFA) [Lactococcus lactis strain FDAARGOS_865]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAAATTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACCATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAACAGGTACCACCTTATATCACGCATCACAAATTCTAAAAGAAGCTGGTGTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B8R081

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.74

85.523

0.639
  comFA/cflA Streptococcus pneumoniae Rx1

52.308

86.86

0.454
  comFA/cflA Streptococcus pneumoniae D39

52.308

86.86

0.454
  comFA/cflA Streptococcus pneumoniae R6

52.308

86.86

0.454
  comFA/cflA Streptococcus pneumoniae TIGR4

52.308

86.86

0.454
  comFA/cflA Streptococcus mitis SK321

52.174

87.082

0.454
  comFA/cflA Streptococcus mitis NCTC 12261

51.407

87.082

0.448
  comFA Bacillus subtilis subsp. subtilis str. 168

36.203

100

0.365