Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   I6G23_RS08855 Genome accession   NZ_CP065735
Coordinates   1505632..1506954 (+) Length   440 a.a.
NCBI ID   WP_021214546.1    Uniprot ID   A0A2X0PIL8
Organism   Lactococcus lactis strain FDAARGOS_866     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1500632..1511954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G23_RS08830 (I6G23_08830) glyQ 1501148..1502101 (-) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -
  I6G23_RS08835 (I6G23_08835) - 1502386..1503132 (-) 747 WP_003130666.1 bifunctional metallophosphatase/5'-nucleotidase -
  I6G23_RS08840 (I6G23_08840) - 1503248..1504267 (-) 1020 WP_003130663.1 DUF475 domain-containing protein -
  I6G23_RS08845 (I6G23_08845) - 1504315..1504812 (-) 498 WP_003130662.1 VanZ family protein -
  I6G23_RS08850 (I6G23_08850) - 1504946..1505575 (-) 630 WP_021214545.1 YigZ family protein -
  I6G23_RS08855 (I6G23_08855) comFA 1505632..1506954 (+) 1323 WP_021214546.1 DEAD/DEAH box helicase Machinery gene
  I6G23_RS13525 comFC 1507197..1507601 (+) 405 WP_003130657.1 ComF family protein Machinery gene
  I6G23_RS08865 (I6G23_08865) - 1507635..1508213 (-) 579 WP_003130655.1 nucleotidyltransferase family protein -
  I6G23_RS08870 (I6G23_08870) - 1508227..1508682 (-) 456 WP_003130653.1 diacylglycerol kinase family protein -
  I6G23_RS08875 (I6G23_08875) ybeY 1508666..1509154 (-) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  I6G23_RS08880 (I6G23_08880) - 1509306..1509785 (-) 480 WP_003130650.1 NUDIX hydrolase -
  I6G23_RS08885 (I6G23_08885) - 1509843..1510811 (-) 969 WP_003130649.1 PhoH family protein -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50187.69 Da        Isoelectric Point: 10.1065

>NTDB_id=513687 I6G23_RS08855 WP_021214546.1 1505632..1506954(+) (comFA) [Lactococcus lactis strain FDAARGOS_866]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDDFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=513687 I6G23_RS08855 WP_021214546.1 1505632..1506954(+) (comFA) [Lactococcus lactis strain FDAARGOS_866]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGATTTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-233)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X0PIL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

38.018

98.636

0.375