Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   I6G43_RS00680 Genome accession   NZ_CP065706
Coordinates   147995..149293 (-) Length   432 a.a.
NCBI ID   WP_038805917.1    Uniprot ID   A0A428FUE1
Organism   Streptococcus oralis strain FDAARGOS_886     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 142995..154293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G43_RS00660 (I6G43_00660) - 143316..145289 (-) 1974 WP_038805914.1 DHH family phosphoesterase -
  I6G43_RS00665 (I6G43_00665) - 145442..146620 (-) 1179 WP_038805915.1 acetyl-CoA C-acetyltransferase -
  I6G43_RS00670 (I6G43_00670) hpf 146708..147256 (-) 549 WP_000599119.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  I6G43_RS00675 (I6G43_00675) comFC/cflB 147336..147998 (-) 663 WP_038805916.1 ComF family protein Machinery gene
  I6G43_RS00680 (I6G43_00680) comFA/cflA 147995..149293 (-) 1299 WP_038805917.1 DEAD/DEAH box helicase Machinery gene
  I6G43_RS00685 (I6G43_00685) - 149350..149985 (+) 636 WP_038805918.1 YigZ family protein -
  I6G43_RS00690 (I6G43_00690) cysK 150141..151067 (+) 927 WP_038805919.1 cysteine synthase A -
  I6G43_RS00695 (I6G43_00695) tsf 151182..152222 (-) 1041 WP_000808060.1 translation elongation factor Ts -
  I6G43_RS00700 (I6G43_00700) rpsB 152301..153080 (-) 780 WP_000268473.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49544.35 Da        Isoelectric Point: 8.0313

>NTDB_id=513180 I6G43_RS00680 WP_038805917.1 147995..149293(-) (comFA/cflA) [Streptococcus oralis strain FDAARGOS_886]
MKVNPNYLGRLFTEKELTEEEREAAVKLQAMRKEKGKLFCQRCDSPILEEWYLPVGAYYCRECLLMKRVRSDQALYYFTQ
EDFPKQDVLKWRGQLTPFQEKVSEGLIRAVEKKEPTLVHAVTGAGKTEMIYQVVAKVIDDGGSVCLASPRIDVCLELYKR
LQNDFACEIALLHGESEPYFRTPLVVATTHQLLKFHHAFDLLIVDEVDAFPYVDNPILYYAVNQCVKEDGLRIFLTATST
DELDKKVRTGELKRLSLPRRFHGNPLIIPKPVWLSDFNRYLDKSQLSPKLKSSIEKQRRTGYPLLIFASEIKKGEKLKEI
LQEQFPNENIGFVSSVTENRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGELLFFHDGLNTSIKKAIKEIKQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=513180 I6G43_RS00680 WP_038805917.1 147995..149293(-) (comFA/cflA) [Streptococcus oralis strain FDAARGOS_886]
ATGAAAGTAAATCCAAATTATCTCGGTCGCTTGTTTACTGAGAAAGAATTAACCGAAGAAGAACGAGAGGCAGCAGTGAA
ACTGCAAGCAATGAGAAAAGAGAAGGGGAAACTATTTTGTCAACGTTGTGACAGTCCTATTTTGGAAGAATGGTATTTGC
CTGTTGGTGCTTACTATTGCAGGGAGTGCTTGCTGATGAAGCGAGTCAGGAGTGATCAAGCTTTATACTATTTTACGCAG
GAGGATTTTCCGAAACAAGATGTTCTTAAATGGCGTGGTCAATTGACTCCTTTTCAAGAGAAGGTATCGGAGGGATTGAT
TCGAGCAGTCGAAAAGAAAGAACCGACCTTAGTTCATGCTGTCACGGGAGCTGGAAAGACAGAGATGATTTATCAAGTTG
TAGCCAAAGTGATTGACGATGGTGGTTCGGTGTGTTTGGCTAGTCCTCGAATCGATGTATGTTTGGAATTGTATAAGCGA
CTGCAGAATGACTTTGCTTGTGAGATAGCACTACTTCATGGCGAATCAGAGCCCTATTTTCGAACTCCCTTAGTAGTTGC
AACGACTCACCAGCTGTTAAAATTTCATCATGCTTTTGATTTGCTGATAGTAGATGAAGTAGATGCCTTTCCTTATGTTG
ACAACCCTATACTTTACTACGCTGTAAACCAATGTGTAAAGGAGGATGGTCTGAGAATCTTTTTAACAGCGACATCTACA
GATGAGTTAGATAAGAAGGTTCGCACTGGAGAATTAAAACGATTAAGCTTGCCAAGACGATTTCATGGAAATCCATTGAT
TATTCCAAAGCCAGTTTGGTTATCGGATTTTAATCGTTATTTAGATAAGAGTCAGTTGTCACCTAAGTTAAAGAGCTCCA
TTGAGAAGCAGAGAAGAACAGGTTATCCCTTGTTAATCTTTGCATCAGAAATTAAAAAAGGCGAGAAACTAAAAGAAATA
TTGCAGGAACAGTTTCCAAATGAAAATATCGGCTTTGTTTCCTCTGTCACAGAAAATCGATTAGAGCAGGTGCAAGCTTT
TCGAGATGGAGAGTTGACGATACTGATCAGTACGACAATTTTAGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAAGCTAATCATCGTCTCTTTACCAAGTCTAGCTTGATTCAGATTGGTGGGCGAGTTGGGCGTAGTATGGACAGA
CCAACTGGTGAGTTGCTCTTCTTTCATGATGGATTAAATACTTCCATCAAAAAGGCAATCAAGGAAATCAAGCAGATGAA
CAAGGAGGCGGGATTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-168)

Helicase conserved C-terminal domain (Helicase_C)

(294-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A428FUE1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

89.583

100

0.896
  comFA/cflA Streptococcus mitis SK321

89.12

100

0.891
  comFA/cflA Streptococcus pneumoniae Rx1

88.889

100

0.889
  comFA/cflA Streptococcus pneumoniae D39

88.889

100

0.889
  comFA/cflA Streptococcus pneumoniae R6

88.889

100

0.889
  comFA/cflA Streptococcus pneumoniae TIGR4

88.657

100

0.887
  comFA Lactococcus lactis subsp. cremoris KW2

51.1

94.676

0.484
  comFA Latilactobacillus sakei subsp. sakei 23K

38.249

100

0.384