Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiA   Type   Regulator
Locus tag   ST4021_RS06830 Genome accession   NZ_CP065493
Coordinates   1324006..1325973 (-) Length   655 a.a.
NCBI ID   WP_046206489.1    Uniprot ID   A0A3G6K5C4
Organism   Streptococcus thermophilus strain 4021     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 1326329..1334786 1324006..1325973 flank 356


Gene organization within MGE regions


Location: 1324006..1334786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4021_RS06830 (ST4021_06860) amiA 1324006..1325973 (-) 1968 WP_046206489.1 peptide ABC transporter substrate-binding protein Regulator
  ST4021_RS06835 (ST4021_06865) - 1326329..1327506 (+) 1178 Protein_1292 ISL3 family transposase -
  ST4021_RS06840 (ST4021_06870) amiA3 1327551..1329524 (-) 1974 WP_011681419.1 peptide ABC transporter substrate-binding protein Regulator
  ST4021_RS06845 (ST4021_06875) - 1329829..1330976 (+) 1148 WP_095559415.1 IS3 family transposase -
  ST4021_RS06850 (ST4021_06880) - 1331014..1331198 (-) 185 Protein_1295 IS3 family transposase -
  ST4021_RS06855 - 1331192..1331593 (-) 402 Protein_1296 ABC transporter ATP-binding protein -
  ST4021_RS06860 (ST4021_06895) - 1331588..1331948 (-) 361 Protein_1297 TatD family hydrolase -
  ST4021_RS06865 (ST4021_06900) - 1332294..1333499 (+) 1206 WP_011681420.1 OFA family MFS transporter -

Sequence

Protein


Download         Length: 655 a.a.        Molecular weight: 71731.83 Da        Isoelectric Point: 5.1056

>NTDB_id=510971 ST4021_RS06830 WP_046206489.1 1324006..1325973(-) (amiA) [Streptococcus thermophilus strain 4021]
MKLKKILGITGVALLSVGMLAACSSKSSTSGTTYSNIYGSDPETLDYITSIMGGTKDVLTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADAKSGALYLVQDSIAGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKQEPYWNSKTTYGVLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDSIKFSYSDGSDLESIERSFSDGALSIARVFPTSSNYASVEKKYKDNIFYTEPGASTSVIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQVNGKDGAALALRNLFVPYDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADGQDGLYNAKKAKTEFAKAKEALQGEGVQFPVHLDLPVDQSSKLSVAQAQSLKQTIEKSLGSENVVIDINQMSSDDMQN
ATSNAANAAAEDWDISNGVGWGPDYQDPSTYLDILKTTSSENTKAFNGYDDPNNAAAAQVGLKDYDALIDSAASETTDLN
ARYDRYAQAQAWLEDSSLVIPLTVSNGAAPIISRLTPFTGASIQVGDKGSSYLKYVKSQEKVVTKKEYEQSREKWLKERK
ASNEKAQKDLEKHIK

Nucleotide


Download         Length: 1968 bp        

>NTDB_id=510971 ST4021_RS06830 WP_046206489.1 1324006..1325973(-) (amiA) [Streptococcus thermophilus strain 4021]
ATGAAACTTAAAAAAATTCTTGGAATTACGGGTGTAGCCCTTCTAAGTGTTGGTATGTTAGCGGCTTGTTCTTCTAAGTC
AAGTACTAGTGGAACGACTTATAGTAATATTTATGGCTCAGATCCAGAAACCTTGGATTATATTACATCAATTATGGGAG
GGACAAAAGATGTTTTGACTAATGGTGTAGATGGTCTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTTACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAGCATGCAGCTGATGCCAAATCTGGAGCCCTTT
ATTTGGTACAAGATTCTATTGCTGGATTGTCTGACTACCTTTCTGGTGCCAATAAAGATTTCTCAAATGTTGGCGTTAAA
GCAATCGACGATTACACTCTGCAATACACACTAAAAAAACAGGAACCATACTGGAACTCTAAGACAACCTATGGTGTGCT
CTTCCCAGTTAACGAAGACTTTCTTAAGAACAAAGGGAAAGATTTTGGTAAGTCAACAGACCCAACCTCTATCCTATATA
ACGGTCCTTTCCTTCTTAAATCTTTGACAGCTAAATCATCTATCGAGTTGACTAAGAATGAGAATTATTGGGATAAGAAA
AACGTGCATTTTGACTCTATCAAATTCTCATATTCTGATGGATCGGATCTAGAATCAATAGAACGTAGTTTCTCTGACGG
CGCTCTCAGCATTGCACGTGTCTTCCCTACGAGTTCAAACTATGCATCGGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGAACCAGGTGCATCTACATCGGTTATTGGTGTAAACATTGACCGCCAAAGCTATAAATTCTCAGCTAAGAAGACA
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTTCGTCAATCAATCAACTTCGCTATTGATCGTAC
AGCCTATCAATCACAAGTAAACGGTAAAGATGGCGCAGCTCTTGCTCTACGTAACCTTTTTGTTCCTTATGATTTTGTCA
GTGCAGGTGATAAGACCTTTGGTGATCTTGTGACAGAAAAAATGAGCTCTTATGGTGATGAGTGGTCTGGTGTTAACTTT
GCAGATGGTCAAGACGGTCTCTACAATGCTAAAAAAGCTAAGACTGAGTTTGCCAAAGCCAAAGAGGCTCTTCAAGGAGA
AGGGGTACAATTCCCAGTTCACTTAGACCTTCCAGTTGATCAAAGTTCTAAACTTAGTGTTGCTCAAGCTCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAAATGTCATCTGATGATATGCAAAAT
GCGACTAGTAATGCGGCAAATGCTGCAGCAGAAGACTGGGATATTTCTAACGGTGTGGGTTGGGGTCCAGATTATCAAGA
TCCATCAACTTATCTTGATATCTTAAAAACAACATCAAGTGAAAATACCAAGGCTTTCAATGGTTATGATGATCCAAACA
ATGCAGCAGCAGCCCAAGTAGGTCTTAAAGATTATGATGCCTTGATTGATTCAGCAGCTTCAGAAACAACAGACCTCAAC
GCTCGTTACGACCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTGTCATTCCATTGACTGTTAGTAACGG
TGCAGCACCGATTATCTCACGTTTGACACCATTTACGGGTGCTAGCATACAGGTGGGTGATAAGGGCTCATCCTACCTCA
AATATGTGAAATCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAGGAAA
GCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACATTAAATAA

Domains

Predicted by InterproScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(75-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G6K5C4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA Streptococcus salivarius strain HSISS4

89.024

100

0.892
  amiA3 Streptococcus thermophilus LMD-9

86.606

100

0.869
  amiA3 Streptococcus thermophilus LMG 18311

86.454

100

0.867