Detailed information NTG0510232

Bioinformatically predicted

Overview

• Name: ruvA
• Type: Machinery gene
• Locus tag: KU544_RS11445
• Genome accession: NZ_CP077897
• Coordinates: 2262115..2262717 (+)
• Length: 200 a.a.
• NCBI ID: WP_000271547.1
• Uniprot ID: Q2YT88
• Organism: Staphylococcus aureus strain 328
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2257115..2267717

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KU544_RS11405 (KU544_11375)	-	2257263..2257382 (+)	120	WP_000840600.1	hypothetical protein	-
KU544_RS11410 (KU544_11380)	mreC	2257581..2258423 (+)	843	WP_000911041.1	rod shape-determining protein MreC	-
KU544_RS11415 (KU544_11385)	mreD	2258423..2258953 (+)	531	WP_225806309.1	rod shape-determining protein MreD	-
KU544_RS11420 (KU544_11390)	rplU	2259189..2259497 (+)	309	WP_000457386.1	50S ribosomal protein L21	-
KU544_RS11425 (KU544_11395)	-	2259503..2259823 (+)	321	WP_000633692.1	ribosomal-processing cysteine protease Prp	-
KU544_RS11430 (KU544_11400)	rpmA	2259835..2260119 (+)	285	WP_000916187.1	50S ribosomal protein L27	-
KU544_RS11435 (KU544_11405)	obgE	2260341..2261633 (+)	1293	WP_000496098.1	GTPase ObgE	-
KU544_RS11440 (KU544_11410)	-	2261646..2262101 (+)	456	WP_000368902.1	ACT domain-containing protein	-
KU544_RS11445 (KU544_11415)	ruvA	2262115..2262717 (+)	603	WP_000271547.1	Holliday junction branch migration protein RuvA	Machinery gene
KU544_RS11450 (KU544_11420)	ruvB	2262749..2263753 (+)	1005	WP_061104859.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
KU544_RS11455 (KU544_11425)	queA	2263755..2264780 (+)	1026	WP_001019180.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
KU544_RS11460 (KU544_11430)	tgt	2264803..2265942 (+)	1140	WP_001112045.1	tRNA guanosine(34) transglycosylase Tgt	-
KU544_RS11465 (KU544_11435)	yajC	2265961..2266221 (+)	261	WP_001160682.1	preprotein translocase subunit YajC	-

Sequence

Protein

• Length: 200 a.a.
• Molecular weight: 22262.46 Da
• Isoelectric Point: 6.0240

>NTDB_id=510232 KU544_RS11445 WP_000271547.1 2262115..2262717(+) (ruvA) [Staphylococcus aureus strain 328]
MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEVLIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGI
GPKSALAILATSTPNEVKRAIENENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS

Nucleotide

• Length: 603 bp

>NTDB_id=510232 KU544_RS11445 WP_000271547.1 2262115..2262717(+) (ruvA) [Staphylococcus aureus strain 328]
ATGTACGCGTATGTCAAAGGTAAGTTAACACATTTATATCCTACACACGTAGTTGTTGAAACTGCTGGTGTTGGTTATGA
AATTCAAACACCAAATTCTTATCGTTTTCAAAAGCATCTAGATCATGAAGTTTTAATTCATACATCTTTAATTGTTCGTG
AAGATGCACAATTATTGTATGGATTTAGTAGTGAAGAAGAGAAAGATATGTTCTTGAGTTTAATTAAAGTTACTGGTATT
GGTCCGAAATCAGCTTTAGCTATTTTAGCGACAAGTACGCCTAATGAAGTAAAACGTGCCATTGAAAATGAAAATGATAC
GTATTTAACTAAATTCCCAGGAATTGGTAAGAAAACGGCAAGACAGATTGTCTTAGATTTAAAAGGTAAAGTGAAAATCA
CTGAAGAAGATAGCGATTCATTATTACAAGTAGACGCTACATCGACGGTGCAAGATCAATTCGTGCAAGAAGCAATGTTA
GCGTTAGAAGCATTAGGTTATTCTAAACGAGAGCTTGCAAAAGTTGAGAAAACGTTAAATAAAAATAAATATGACTCAGT
TGATGAAGCTGTTAAGGCAGGTCTTCAATTAGTTGTATCTTAA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix domain (HHH_5) (71-128)

• RuvA, C-terminal domain (RuvA_C) (156-197)

• RuvA N terminal domain (RuvA_N) (1-61)

3D structure

• AlphaFold DB: Q2YT88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvA	Streptococcus pneumoniae TIGR4	46.231	99.5	0.46
ruvA	Streptococcus pneumoniae R6	45.729	99.5	0.455
ruvA	Streptococcus pneumoniae D39	45.729	99.5	0.455
ruvA	Bacillus subtilis subsp. subtilis str. 168	42.714	99.5	0.425