Detailed information NTG0510031

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: ST4078_RS08825
• Genome accession: NZ_CP065477
• Coordinates: 1693005..1693442 (-)
• Length: 145 a.a.
• NCBI ID: WP_014608742.1
• Uniprot ID: -
• Organism: Streptococcus thermophilus strain 4078
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1688005..1698442

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ST4078_RS08785 (ST4078_08730)	-	1688069..1688731 (-)	663	WP_011681679.1	CPBP family intramembrane glutamic endopeptidase	-
ST4078_RS08790 (ST4078_08735)	-	1688828..1689271 (-)	444	WP_011681680.1	hypothetical protein	-
ST4078_RS08795	-	1689345..1689683 (-)	339	WP_224103197.1	CPBP family intramembrane glutamic endopeptidase	-
ST4078_RS08800	-	1689727..1690002 (-)	276	WP_011681681.1	hypothetical protein	-
ST4078_RS08805 (ST4078_08745)	-	1690014..1690211 (-)	198	WP_011681682.1	helix-turn-helix transcriptional regulator	-
ST4078_RS08810 (ST4078_08750)	-	1690460..1691653 (-)	1194	WP_014608740.1	acetate kinase	-
ST4078_RS08815 (ST4078_08755)	comYH	1691709..1692665 (-)	957	WP_014608741.1	class I SAM-dependent methyltransferase	Machinery gene
ST4078_RS08820 (ST4078_08760)	comGG	1692710..1693027 (-)	318	WP_002952035.1	competence type IV pilus minor pilin ComGG	-
ST4078_RS08825 (ST4078_08765)	comYF	1693005..1693442 (-)	438	WP_014608742.1	competence type IV pilus minor pilin ComGF	Machinery gene
ST4078_RS08830 (ST4078_08770)	comGE	1693426..1693719 (-)	294	WP_011226625.1	competence type IV pilus minor pilin ComGE	-
ST4078_RS08835 (ST4078_08775)	comYD	1693691..1694119 (-)	429	WP_011226626.1	competence type IV pilus minor pilin ComGD	Machinery gene
ST4078_RS08840 (ST4078_08780)	comYC	1694079..1694405 (-)	327	WP_002946126.1	competence type IV pilus major pilin ComGC	Machinery gene
ST4078_RS08845 (ST4078_08785)	comYB	1694402..1695502 (-)	1101	WP_116920398.1	competence type IV pilus assembly protein ComGB	Machinery gene
ST4078_RS08850 (ST4078_08790)	comGA/cglA/cilD	1695384..1696325 (-)	942	WP_014608745.1	competence type IV pilus ATPase ComGA	Machinery gene
ST4078_RS08855 (ST4078_08795)	-	1696406..1696768 (-)	363	WP_014608746.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16505.05 Da
• Isoelectric Point: 8.9784

>NTDB_id=510031 ST4078_RS08825 WP_014608742.1 1693005..1693442(-) (comYF) [Streptococcus thermophilus strain 4078]
MYLSSKLRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVRQQENQDWLLFVQQMEAELEGCQLVKVEWNKLYVKKD
NQDLAFGLSSASDFRKTNADGRGYQPMLFDVKSSKVSQKNQIVTIQVTLKNELQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=510031 ST4078_RS08825 WP_014608742.1 1693005..1693442(-) (comYF) [Streptococcus thermophilus strain 4078]
ATGTATCTAAGCAGTAAGCTGCGTGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTCATTGCTGGCTCAGTTCA
AGTTTATCAAGGGTTAACCACTGTACTTGTGAGTAATGTTAAGCAGGTAAGACAACAAGAAAACCAAGATTGGCTTCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGATGTCAGTTGGTCAAGGTAGAGTGGAACAAACTCTATGTCAAGAAGGAT
AATCAAGACTTGGCTTTTGGTCTTTCAAGTGCCTCTGATTTTCGAAAAACAAATGCTGATGGTCGAGGTTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTAAGGTAAGTCAAAAGAACCAGATAGTTACCATCCAAGTGACTCTTAAAAATGAACTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.865	97.241	0.572
comYF	Streptococcus mutans UA159	58.156	97.241	0.566
comGF/cglF	Streptococcus pneumoniae D39	47.857	96.552	0.462
comGF/cglF	Streptococcus pneumoniae Rx1	47.857	96.552	0.462
comGF/cglF	Streptococcus pneumoniae R6	47.857	96.552	0.462
comGF/cglF	Streptococcus pneumoniae TIGR4	47.857	96.552	0.462
comGF/cglF	Streptococcus mitis NCTC 12261	46.099	97.241	0.448
comGF/cglF	Streptococcus mitis SK321	45.39	97.241	0.441
comGF	Lactococcus lactis subsp. cremoris KW2	46.043	95.862	0.441