Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   I5M77_RS15685 Genome accession   NZ_CP065369
Coordinates   3288923..3289501 (+) Length   192 a.a.
NCBI ID   WP_015297396.1    Uniprot ID   A0A243VIJ8
Organism   Vibrio parahaemolyticus strain 20-082A3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3283923..3294501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5M77_RS15670 (I5M77_15670) oxyR 3284246..3285154 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  I5M77_RS15675 (I5M77_15675) - 3285210..3287780 (-) 2571 WP_005480476.1 penicillin-binding protein 1A -
  I5M77_RS15680 (I5M77_15680) pilM 3287923..3288939 (+) 1017 WP_025793368.1 type IV pilus biogenesis protein PilM Machinery gene
  I5M77_RS15685 (I5M77_15685) pilN 3288923..3289501 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  I5M77_RS15690 (I5M77_15690) pilO 3289494..3290087 (+) 594 WP_025793367.1 type 4a pilus biogenesis protein PilO Machinery gene
  I5M77_RS15695 (I5M77_15695) pilP 3290077..3290592 (+) 516 WP_023585576.1 pilus assembly protein PilP Machinery gene
  I5M77_RS15700 (I5M77_15700) pilQ 3290624..3292372 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  I5M77_RS15705 (I5M77_15705) aroK 3292561..3293079 (+) 519 WP_005381319.1 shikimate kinase AroK -
  I5M77_RS15710 (I5M77_15710) aroB 3293111..3294211 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.95 Da        Isoelectric Point: 6.9944

>NTDB_id=508891 I5M77_RS15685 WP_015297396.1 3288923..3289501(+) (pilN) [Vibrio parahaemolyticus strain 20-082A3]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=508891 I5M77_RS15685 WP_015297396.1 3288923..3289501(+) (pilN) [Vibrio parahaemolyticus strain 20-082A3]
GTGCTGTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGAGTCATGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTACCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCTCGGCTAGCAACCATGCTCGATAACATGGAGCGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTCGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTCAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243VIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594