Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   I5M77_RS15680 Genome accession   NZ_CP065369
Coordinates   3287923..3288939 (+) Length   338 a.a.
NCBI ID   WP_025793368.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 20-082A3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3282923..3293939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5M77_RS15665 (I5M77_15665) - 3283347..3284075 (-) 729 WP_015297397.1 redoxin family protein -
  I5M77_RS15670 (I5M77_15670) oxyR 3284246..3285154 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  I5M77_RS15675 (I5M77_15675) - 3285210..3287780 (-) 2571 WP_005480476.1 penicillin-binding protein 1A -
  I5M77_RS15680 (I5M77_15680) pilM 3287923..3288939 (+) 1017 WP_025793368.1 type IV pilus biogenesis protein PilM Machinery gene
  I5M77_RS15685 (I5M77_15685) pilN 3288923..3289501 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  I5M77_RS15690 (I5M77_15690) pilO 3289494..3290087 (+) 594 WP_025793367.1 type 4a pilus biogenesis protein PilO Machinery gene
  I5M77_RS15695 (I5M77_15695) pilP 3290077..3290592 (+) 516 WP_023585576.1 pilus assembly protein PilP Machinery gene
  I5M77_RS15700 (I5M77_15700) pilQ 3290624..3292372 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  I5M77_RS15705 (I5M77_15705) aroK 3292561..3293079 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37780.32 Da        Isoelectric Point: 6.8323

>NTDB_id=508890 I5M77_RS15680 WP_025793368.1 3287923..3288939(+) (pilM) [Vibrio parahaemolyticus strain 20-082A3]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMMAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=508890 I5M77_RS15680 WP_025793368.1 3287923..3288939(+) (pilM) [Vibrio parahaemolyticus strain 20-082A3]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGATGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ATCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.071

99.704

0.479