Detailed information NTG0050889

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DGO_RS11710
• Genome accession: NC_017790
• Coordinates: 2466045..2468708 (+)
• Length: 887 a.a.
• NCBI ID: WP_014685734.1
• Uniprot ID: H8GZL3
• Organism: Deinococcus gobiensis I-0
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2461045..2473708

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DGO_RS11685 (DGo_CA2319)	-	2462167..2463192 (-)	1026	WP_014685729.1	M42 family metallopeptidase	-
DGO_RS11690 (DGo_CA2320)	-	2463234..2463647 (-)	414	WP_043802199.1	isochorismatase family protein	-
DGO_RS11695 (DGo_CA2321)	-	2463739..2464530 (-)	792	WP_014685731.1	enoyl-ACP reductase FabI	-
DGO_RS11700 (DGo_CA2322)	pgeF	2464636..2465379 (+)	744	WP_043802202.1	peptidoglycan editing factor PgeF	-
DGO_RS11705 (DGo_CA2323)	-	2465376..2465888 (+)	513	WP_014685733.1	YqeG family HAD IIIA-type phosphatase	-
DGO_RS11710 (DGo_CA2324)	pilB	2466045..2468708 (+)	2664	WP_014685734.1	type II/IV secretion system protein	Machinery gene
DGO_RS11715 (DGo_CA2325)	pilT	2468834..2470069 (+)	1236	WP_043802203.1	type IV pilus twitching motility protein PilT	Machinery gene
DGO_RS11720 (DGo_CA2326)	-	2470723..2472627 (+)	1905	WP_014685736.1	CASTOR/POLLUX-related putative ion channel	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 96375.37 Da
• Isoelectric Point: 4.8403

>NTDB_id=50889 DGO_RS11710 WP_014685734.1 2466045..2468708(+) (pilB) [Deinococcus gobiensis I-0]
MALSIGDRRLGAILLEQGYVNDTDLQKALARHAEVGGRLADVLIDSGVVAEKRIARAIEEALGIPLVNLMAVTPEPEALR
AVQPQTAMSVQAFPFALDGGTLRVALVDPLSSLAIEALEDDSGLNIEPYQALREEVLWCLATHYPELRLQAQAPSEEAGG
TRAQLGQRLIAAGLINDAQLQVALDIQQQTGVALGGVLIEQGLITEAQLYTQLAEQAGVPFLPEPRGFQPTEDVLGSMLR
ADALRLSAVPIDETAQGVTVITSDLRKRADIAALVGQNVQFMLAQPRDIERLIEEFYPQRGRLGEQLVQQGTLSRAQLRE
ALHVQARDSKVKPLGEVILDLGFATAEEVDGALQKQNVGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNPPDA
KVALMIPESTARRYVVVPVRIQGDSLVVAMKDPRNVFALDDLKLLTGREIVPAVMAEKDIVRLIERYFGNQDMANLNQRL
VKESAKRETAVDLDLSAGLDDNAVVRVVDNLIREAALQEASDIHIEPTETSVRVRYRIDGVLREQAELPKGAAQSLLARI
KIMGHLDIAERRIPQDGRVRFRKGSIDIDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSEYNIQRYSDVIEKPNGI
FLVTGPTGSGKSFTSFSTLKRIARPEKNTTTIEDPVEYEIPGINQSQVNNAAGMTFARALRAFLRQDPDIIFVGEIRDTE
TAEIAVEAALTGHLVLATLHTNDAPGAVTRLEEMGVEPFNISAAVAGVLAQRLVRRVCTECKAPTNADPDVLRRLGIAER
DLRGAQLMRGAGCPRCGGTGYKGRMGIHELMVVDEPLRAAIGAGRSSAEIRDVAMEKSGMKTLRQDGIEKALQGLTTLEE
VLAVTSS

Nucleotide

• Length: 2664 bp

>NTDB_id=50889 DGO_RS11710 WP_014685734.1 2466045..2468708(+) (pilB) [Deinococcus gobiensis I-0]
GTGGCTCTTTCGATAGGTGACCGGCGCTTGGGTGCCATCTTGCTCGAACAGGGGTACGTCAACGACACGGACCTGCAAAA
GGCCCTGGCGCGGCACGCCGAGGTGGGGGGCCGCCTGGCCGACGTGCTGATCGACTCGGGTGTGGTGGCCGAGAAGCGCA
TCGCGCGGGCCATCGAGGAGGCGCTGGGCATCCCGCTCGTGAACCTCATGGCCGTGACCCCCGAGCCCGAGGCGCTGCGG
GCGGTGCAGCCGCAGACCGCCATGAGTGTCCAGGCCTTTCCCTTCGCCCTCGACGGCGGAACGCTGCGGGTGGCGCTGGT
GGACCCGCTGTCCAGCCTCGCCATCGAGGCGCTGGAGGACGACAGCGGCCTGAACATCGAGCCGTATCAGGCGCTGCGCG
AGGAGGTGCTGTGGTGCCTCGCCACGCACTACCCCGAACTGCGGCTTCAGGCCCAGGCACCCAGCGAGGAGGCCGGCGGG
ACGCGCGCCCAGCTCGGCCAGCGCCTCATTGCCGCCGGGCTGATCAACGACGCGCAGCTTCAGGTGGCGCTCGACATCCA
GCAGCAGACCGGCGTCGCGCTGGGCGGCGTGCTCATCGAGCAGGGCCTGATCACCGAGGCCCAGCTCTACACGCAGCTCG
CCGAGCAGGCGGGCGTGCCCTTCCTGCCCGAACCGCGCGGTTTCCAGCCGACCGAGGACGTGCTGGGCAGCATGCTGCGC
GCCGACGCGCTGCGGCTCTCGGCGGTGCCCATCGACGAGACGGCGCAGGGCGTGACCGTCATCACCAGCGACCTGCGCAA
GCGCGCCGACATCGCGGCGCTGGTGGGCCAGAACGTGCAGTTCATGCTGGCGCAGCCGCGCGACATCGAGCGGCTCATCG
AGGAGTTCTACCCGCAGCGCGGCCGCCTGGGCGAACAGCTCGTGCAGCAGGGCACGCTGTCCCGCGCGCAGCTGCGCGAG
GCGCTGCACGTCCAGGCCCGCGACAGCAAGGTCAAGCCGCTGGGCGAGGTGATCCTGGACCTGGGGTTCGCCACGGCCGA
GGAAGTGGACGGCGCGCTGCAAAAGCAGAACGTGGGCGGCGGCCGCCTGGAAGACACGCTGGTGCAGTCGGGCAAGCTCT
CGCCCGAGATGCTGGCGCGCTCGCTGGCCGCGCAGCTCGGCTACGAGTTCCTCGACCCGGTCCAGAACCCGCCCGACGCC
AAGGTCGCGCTGATGATCCCCGAGAGCACCGCGCGGCGCTACGTGGTCGTGCCGGTGCGCATCCAGGGCGACAGCCTCGT
CGTCGCCATGAAGGACCCGCGCAACGTGTTCGCGCTCGACGACCTCAAGCTCCTCACCGGCCGCGAGATCGTGCCCGCCG
TGATGGCGGAGAAGGACATCGTCCGCCTGATCGAACGCTATTTCGGCAACCAGGACATGGCGAACCTCAACCAGCGCCTC
GTCAAGGAGAGCGCCAAGCGCGAGACGGCGGTGGACCTCGACCTCTCGGCGGGCCTGGACGACAACGCCGTGGTCCGGGT
CGTGGACAACCTGATCCGCGAGGCGGCGTTGCAGGAAGCCAGCGATATCCACATCGAACCGACCGAGACCTCCGTGCGGG
TGCGCTACCGCATCGACGGCGTACTGCGCGAGCAGGCCGAGCTGCCCAAGGGCGCGGCCCAGAGCCTGCTGGCCCGCATC
AAGATCATGGGGCACCTCGACATCGCCGAGCGCCGCATTCCGCAGGACGGCCGCGTGCGCTTCCGCAAGGGCAGCATCGA
CATCGACCTGCGCCTCTCGACCCTGCCCACCGTCTACGGCGAGAAGGCCGTGATGCGTCTGCTGCAGAAGGCCAGCAACA
TCCCCGAGGTCGAGCAGCTCGGCTTTTCCGAATACAACATCCAGCGCTACTCGGACGTGATCGAGAAGCCCAACGGCATC
TTCCTGGTGACGGGACCCACAGGGTCGGGCAAGTCCTTCACCAGTTTTTCCACCCTCAAGCGCATCGCGCGCCCCGAAAA
GAACACCACGACCATCGAGGACCCGGTCGAGTACGAGATTCCGGGCATTAACCAGTCGCAGGTGAACAACGCGGCCGGCA
TGACCTTCGCCCGCGCCCTGCGCGCCTTCCTGCGTCAGGACCCTGACATCATCTTCGTGGGGGAAATCCGCGACACCGAG
ACGGCCGAGATCGCGGTCGAGGCGGCGCTCACCGGCCACCTCGTGCTGGCGACCCTGCACACCAACGACGCCCCCGGCGC
GGTGACCCGTCTGGAGGAGATGGGCGTGGAGCCCTTCAACATCTCGGCCGCCGTGGCGGGGGTGCTCGCGCAGCGCCTCG
TGCGCCGGGTCTGCACCGAGTGCAAGGCCCCCACCAACGCCGACCCCGACGTGCTGCGCCGCCTGGGCATCGCCGAGCGC
GACCTGCGCGGGGCCCAGCTCATGCGCGGGGCGGGCTGCCCTCGCTGCGGGGGCACCGGCTACAAGGGCCGCATGGGCAT
CCACGAACTGATGGTCGTGGACGAGCCGCTGCGCGCCGCCATCGGGGCCGGGCGTTCCTCGGCCGAGATCCGCGACGTGG
CGATGGAAAAGAGCGGCATGAAGACGCTACGTCAGGACGGCATCGAGAAGGCGCTGCAAGGCCTGACCACCCTCGAAGAA
GTGCTGGCCGTCACCTCCAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (511-776)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (198-301)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (367-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

3D structure

• AlphaFold DB: H8GZL3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	79.19	100	0.794
pilF	Thermus thermophilus HB27	56.292	100	0.565