Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   I3J23_RS17830 Genome accession   NZ_CP065159
Coordinates   3499606..3500505 (+) Length   299 a.a.
NCBI ID   WP_088037136.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GXU-1     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3494606..3505505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3J23_RS17810 (I3J23_17810) - 3495297..3497000 (+) 1704 WP_048367242.1 hypothetical protein -
  I3J23_RS17815 (I3J23_17815) - 3496997..3497278 (+) 282 WP_003154284.1 FlhB-like flagellar biosynthesis protein -
  I3J23_RS17820 (I3J23_17820) sucC 3497453..3498610 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  I3J23_RS17825 (I3J23_17825) sucD 3498639..3499541 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  I3J23_RS17830 (I3J23_17830) dprA 3499606..3500505 (+) 900 WP_088037136.1 DNA-processing protein DprA Machinery gene
  I3J23_RS17835 (I3J23_17835) topA 3500687..3502762 (+) 2076 WP_003154274.1 type I DNA topoisomerase -
  I3J23_RS17840 (I3J23_17840) trmFO 3502827..3504134 (+) 1308 WP_072588509.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  I3J23_RS17845 (I3J23_17845) xerC 3504204..3505121 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32888.07 Da        Isoelectric Point: 8.3387

>NTDB_id=507576 I3J23_RS17830 WP_088037136.1 3499606..3500505(+) (dprA) [Bacillus amyloliquefaciens strain GXU-1]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFFSDPHPLTVLSEGKTAPEAIFREIERKDPELDEVLSDYRRKGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTPLLEKGRKIGIVGTRKPTEDGIKAAGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRVKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYCGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=507576 I3J23_RS17830 WP_088037136.1 3499606..3500505(+) (dprA) [Bacillus amyloliquefaciens strain GXU-1]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCACTCTCTGTCTTTTTTTTCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGGATCCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCAAAGGAATTACTGTCATT
CCGATTTCATCAAGCCGCTACCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGGAACAC
GCCGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGCTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGATTGTCTCATAAG
GCGAGCATCAGGGTAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGACTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCCCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTGCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

69.9

100

0.699
  dprA Lactococcus lactis subsp. cremoris KW2

40.143

93.311

0.375