Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I3J23_RS02305 Genome accession   NZ_CP065159
Coordinates   440553..441623 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GXU-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 435553..446623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3J23_RS02255 (I3J23_02255) - 435613..436197 (-) 585 WP_046559873.1 signal peptidase I -
  I3J23_RS02260 (I3J23_02260) tapA 436169..436840 (-) 672 WP_046559874.1 amyloid fiber anchoring/assembly protein TapA -
  I3J23_RS02265 (I3J23_02265) - 437099..437428 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  I3J23_RS02270 (I3J23_02270) - 437468..437647 (-) 180 WP_003153093.1 YqzE family protein -
  I3J23_RS02275 (I3J23_02275) comGG 437704..438081 (-) 378 WP_046559875.1 competence type IV pilus minor pilin ComGG Machinery gene
  I3J23_RS02280 (I3J23_02280) comGF 438082..438582 (-) 501 WP_224979425.1 competence type IV pilus minor pilin ComGF -
  I3J23_RS02285 (I3J23_02285) comGE 438491..438805 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  I3J23_RS02290 (I3J23_02290) comGD 438789..439226 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  I3J23_RS02295 (I3J23_02295) comGC 439216..439524 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  I3J23_RS02300 (I3J23_02300) comGB 439529..440566 (-) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  I3J23_RS02305 (I3J23_02305) comGA 440553..441623 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  I3J23_RS02310 (I3J23_02310) - 441815..442765 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  I3J23_RS02315 (I3J23_02315) - 442911..444212 (+) 1302 WP_014305416.1 hemolysin family protein -
  I3J23_RS02320 (I3J23_02320) - 444251..445081 (-) 831 WP_003153080.1 STAS domain-containing protein -
  I3J23_RS02330 (I3J23_02330) - 445287..445667 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  I3J23_RS02335 (I3J23_02335) - 445903..446148 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=507515 I3J23_RS02305 WP_003153083.1 440553..441623(-) (comGA) [Bacillus amyloliquefaciens strain GXU-1]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=507515 I3J23_RS02305 WP_003153083.1 440553..441623(-) (comGA) [Bacillus amyloliquefaciens strain GXU-1]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374