NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	IRJ20_RS02390	Genome accession	NZ_CP065137
Coordinates	458267..458881 (-)	Length	204 a.a.
NCBI ID	WP_015240245.1	Uniprot ID	-
Organism	Bacillus sp. A1(2020)
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 453267..463881

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IRJ20_RS02360	rpsT	453570..453836 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
IRJ20_RS02365	holA	453853..454894 (-)	1042	Protein_467	DNA polymerase III subunit delta	-
IRJ20_RS02370	-	454934..455085 (-)	152	Protein_468	hypothetical protein	-
IRJ20_RS02375	-	455126..455260 (+)	135	WP_003152870.1	YqzM family protein	-
IRJ20_RS02380	comEC	455279..457630 (-)	2352	WP_039254415.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
IRJ20_RS02385	-	457631..458200 (-)	570	WP_003152868.1	ComE operon protein 2	-
IRJ20_RS02390	comEA	458267..458881 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
IRJ20_RS02395	comER	458940..459761 (+)	822	WP_242750419.1	late competence protein ComER	-
IRJ20_RS02400	-	459830..460567 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
IRJ20_RS02405	rsfS	460564..460920 (-)	357	WP_007408260.1	ribosome silencing factor	-
IRJ20_RS02410	yqeK	460938..461498 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
IRJ20_RS02415	-	461488..462057 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
IRJ20_RS02420	yhbY	462068..462358 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
IRJ20_RS02425	aroE	462352..463194 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22209.32 Da Isoelectric Point: 5.5211

                                >NTDB_id=507202 IRJ20_RS02390 WP_015240245.1 458267..458881(-) (comEA) [Bacillus sp. A1(2020)]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=507202 IRJ20_RS02390 WP_015240245.1 458267..458881(-) (comEA) [Bacillus sp. A1(2020)]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0507202