Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I0P33_RS02990 Genome accession   NZ_CP065100
Coordinates   638862..639842 (-) Length   326 a.a.
NCBI ID   WP_001055644.1    Uniprot ID   -
Organism   Salmonella enterica subsp. enterica strain SENT0058     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 633862..644842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0P33_RS02960 (I0P33_02940) endA 634319..635026 (+) 708 WP_001650639.1 deoxyribonuclease I -
  I0P33_RS02965 (I0P33_02945) rsmE 635103..635834 (+) 732 WP_001222489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I0P33_RS02970 (I0P33_02950) gshB 635854..636801 (+) 948 WP_000593243.1 glutathione synthase -
  I0P33_RS02975 (I0P33_02955) - 637017..637580 (+) 564 WP_001053171.1 YqgE/AlgH family protein -
  I0P33_RS02980 (I0P33_02960) ruvX 637580..637996 (+) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  I0P33_RS02985 (I0P33_02965) - 638043..638729 (-) 687 WP_023260784.1 IclR family transcriptional regulator -
  I0P33_RS02990 (I0P33_02970) pilT 638862..639842 (-) 981 WP_001055644.1 type IV pilus twitching motility protein PilT Machinery gene
  I0P33_RS02995 (I0P33_02975) - 639860..640564 (+) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  I0P33_RS03000 (I0P33_02980) - 640583..641149 (+) 567 WP_001094848.1 YggT family protein -
  I0P33_RS03005 (I0P33_02985) yggU 641146..641436 (+) 291 WP_001277205.1 DUF167 family protein YggU -
  I0P33_RS03010 (I0P33_02990) - 641444..642037 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  I0P33_RS03015 (I0P33_02995) hemW 642030..643166 (+) 1137 WP_058108546.1 radical SAM family heme chaperone HemW -
  I0P33_RS03020 (I0P33_03000) - 643257..644264 (-) 1008 WP_061423671.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36139.31 Da        Isoelectric Point: 7.5356

>NTDB_id=506979 I0P33_RS02990 WP_001055644.1 638862..639842(-) (pilT) [Salmonella enterica subsp. enterica strain SENT0058]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQEGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=506979 I0P33_RS02990 WP_001055644.1 638862..639842(-) (pilT) [Salmonella enterica subsp. enterica strain SENT0058]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACCACGTTGGCGGCAATGGTGGGACATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGAGGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362
  pilU Pseudomonas stutzeri DSM 10701

35.758

100

0.362