Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IXY25_RS00720 Genome accession   NZ_CP064846
Coordinates   129450..130520 (+) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain BZR 86     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 124450..135520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IXY25_RS00690 (IXY25_00680) - 124924..125169 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  IXY25_RS00695 (IXY25_00685) - 125406..125786 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  IXY25_RS00705 (IXY25_00695) - 125992..126822 (+) 831 WP_025852928.1 STAS domain-containing protein -
  IXY25_RS00710 (IXY25_00700) - 126861..128162 (-) 1302 WP_014305416.1 hemolysin family protein -
  IXY25_RS00715 (IXY25_00705) - 128308..129258 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  IXY25_RS00720 (IXY25_00710) comGA 129450..130520 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  IXY25_RS00725 (IXY25_00715) comGB 130507..131544 (+) 1038 WP_024085602.1 competence type IV pilus assembly protein ComGB Machinery gene
  IXY25_RS00730 (IXY25_00720) comGC 131591..131857 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  IXY25_RS00735 (IXY25_00725) comGD 131847..132284 (+) 438 WP_025852922.1 competence type IV pilus minor pilin ComGD Machinery gene
  IXY25_RS00740 (IXY25_00730) comGE 132268..132582 (+) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  IXY25_RS00745 (IXY25_00735) comGF 132491..132991 (+) 501 WP_226565836.1 competence type IV pilus minor pilin ComGF -
  IXY25_RS00750 (IXY25_00740) comGG 132992..133369 (+) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  IXY25_RS00755 (IXY25_00745) - 133426..133605 (+) 180 WP_003153093.1 YqzE family protein -
  IXY25_RS00760 (IXY25_00750) - 133645..133974 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  IXY25_RS00765 (IXY25_00755) tapA 134233..134904 (+) 672 WP_025852919.1 amyloid fiber anchoring/assembly protein TapA -
  IXY25_RS00770 (IXY25_00760) sipW 134876..135460 (+) 585 WP_025852917.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=505248 IXY25_RS00720 WP_003153083.1 129450..130520(+) (comGA) [Bacillus velezensis strain BZR 86]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=505248 IXY25_RS00720 WP_003153083.1 129450..130520(+) (comGA) [Bacillus velezensis strain BZR 86]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374