Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   IXY25_RS00725 Genome accession   NZ_CP064846
Coordinates   130507..131544 (+) Length   345 a.a.
NCBI ID   WP_024085602.1    Uniprot ID   -
Organism   Bacillus velezensis strain BZR 86     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 125507..136544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IXY25_RS00705 (IXY25_00695) - 125992..126822 (+) 831 WP_025852928.1 STAS domain-containing protein -
  IXY25_RS00710 (IXY25_00700) - 126861..128162 (-) 1302 WP_014305416.1 hemolysin family protein -
  IXY25_RS00715 (IXY25_00705) - 128308..129258 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  IXY25_RS00720 (IXY25_00710) comGA 129450..130520 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  IXY25_RS00725 (IXY25_00715) comGB 130507..131544 (+) 1038 WP_024085602.1 competence type IV pilus assembly protein ComGB Machinery gene
  IXY25_RS00730 (IXY25_00720) comGC 131591..131857 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  IXY25_RS00735 (IXY25_00725) comGD 131847..132284 (+) 438 WP_025852922.1 competence type IV pilus minor pilin ComGD Machinery gene
  IXY25_RS00740 (IXY25_00730) comGE 132268..132582 (+) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  IXY25_RS00745 (IXY25_00735) comGF 132491..132991 (+) 501 WP_226565836.1 competence type IV pilus minor pilin ComGF -
  IXY25_RS00750 (IXY25_00740) comGG 132992..133369 (+) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  IXY25_RS00755 (IXY25_00745) - 133426..133605 (+) 180 WP_003153093.1 YqzE family protein -
  IXY25_RS00760 (IXY25_00750) - 133645..133974 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  IXY25_RS00765 (IXY25_00755) tapA 134233..134904 (+) 672 WP_025852919.1 amyloid fiber anchoring/assembly protein TapA -
  IXY25_RS00770 (IXY25_00760) sipW 134876..135460 (+) 585 WP_025852917.1 signal peptidase I SipW -
  IXY25_RS00775 (IXY25_00765) tasA 135524..136309 (+) 786 WP_003153102.1 biofilm matrix protein TasA -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39618.91 Da        Isoelectric Point: 9.5658

>NTDB_id=505249 IXY25_RS00725 WP_024085602.1 130507..131544(+) (comGB) [Bacillus velezensis strain BZR 86]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYEVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPYYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=505249 IXY25_RS00725 WP_024085602.1 130507..131544(+) (comGB) [Bacillus velezensis strain BZR 86]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACGAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCAGAGAAACACGGAGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTCATTGTTGTGAAAATACCCCTCTTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTATTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614