Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HWI32_RS06255 Genome accession   NZ_CP064568
Coordinates   1314365..1315339 (+) Length   324 a.a.
NCBI ID   WP_001831109.1    Uniprot ID   A0A9Q5JKD6
Organism   Staphylococcus epidermidis strain UMCG359     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1309365..1320339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI32_RS06220 (HWI32_06200) rpmG 1309845..1309994 (+) 150 WP_001830957.1 50S ribosomal protein L33 -
  HWI32_RS06225 (HWI32_06205) - 1310177..1310728 (+) 552 WP_001831001.1 5-formyltetrahydrofolate cyclo-ligase -
  HWI32_RS06230 (HWI32_06210) - 1310729..1312189 (+) 1461 WP_001832757.1 rhomboid family intramembrane serine protease -
  HWI32_RS06235 (HWI32_06215) - 1312173..1312373 (+) 201 WP_001831138.1 YqgQ family protein -
  HWI32_RS06240 (HWI32_06220) - 1312373..1313359 (+) 987 WP_001831113.1 ROK family glucokinase -
  HWI32_RS06245 (HWI32_06225) - 1313359..1313685 (+) 327 WP_001831234.1 MTH1187 family thiamine-binding protein -
  HWI32_RS06250 (HWI32_06230) - 1313685..1314308 (+) 624 WP_001831016.1 MBL fold metallo-hydrolase -
  HWI32_RS06255 (HWI32_06235) comGA 1314365..1315339 (+) 975 WP_001831109.1 competence type IV pilus ATPase ComGA Machinery gene
  HWI32_RS06260 (HWI32_06240) comGB 1315311..1316378 (+) 1068 WP_001831131.1 competence type IV pilus assembly protein ComGB Machinery gene
  HWI32_RS06265 (HWI32_06245) comGC 1316396..1316713 (+) 318 WP_001831271.1 competence type IV pilus major pilin ComGC Machinery gene
  HWI32_RS06270 (HWI32_06250) comGD 1316703..1317140 (+) 438 WP_001831025.1 competence type IV pilus minor pilin ComGD -
  HWI32_RS06275 (HWI32_06255) - 1317127..1317420 (+) 294 WP_001831116.1 hypothetical protein -
  HWI32_RS06280 (HWI32_06260) - 1317344..1317835 (+) 492 WP_002440043.1 competence type IV pilus minor pilin ComGF -
  HWI32_RS06285 (HWI32_06265) - 1318000..1318512 (+) 513 WP_002440040.1 shikimate kinase -
  HWI32_RS06290 (HWI32_06270) gcvT 1318730..1319821 (+) 1092 WP_001831107.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 37330.44 Da        Isoelectric Point: 8.8174

>NTDB_id=503157 HWI32_RS06255 WP_001831109.1 1314365..1315339(+) (comGA) [Staphylococcus epidermidis strain UMCG359]
MKILFEQIINHAIKQEASDIHFIPCEEHTIIKLRIKDELTIYDRLSFPIYKKLLIYMKFQSGLDVSTQHRAQSGRYSYKM
KHLYYLRISTLPLSLGNESCVIRIVPQYFQTTRESYEFKDFKHFMKKKQGLLLFSGPTGSGKSTLMYQMVLYAHQKLNLN
VISVEDPVEQILNGITQISVNEKAGINYESSFKAILRCDPDVILIGEIRDSTVAKYVIQASLSGHLVLSTMHANDCKGAL
LRLLEMGISVQELCQAINLISNQRLITTTTNYRQLVSELMFQSQINYFFEHNHSLPKNFTKLATHLNKMSKEGVICEEVV
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=503157 HWI32_RS06255 WP_001831109.1 1314365..1315339(+) (comGA) [Staphylococcus epidermidis strain UMCG359]
TTGAAAATTTTATTTGAACAGATTATCAATCATGCAATTAAGCAAGAAGCAAGTGACATACACTTTATACCATGTGAGGA
ACACACTATAATTAAGCTAAGAATTAAAGATGAGCTCACAATATATGATAGACTTTCATTTCCAATTTATAAAAAATTAC
TTATATACATGAAGTTTCAATCTGGCTTAGATGTATCAACACAGCATAGAGCACAAAGTGGTAGATATAGTTATAAGATG
AAGCATTTATATTATTTAAGAATCTCTACATTACCTTTATCATTAGGCAATGAAAGTTGTGTAATTCGTATAGTACCTCA
ATACTTCCAAACTACACGAGAATCATATGAATTTAAAGATTTTAAACACTTTATGAAAAAGAAACAAGGTCTACTTTTGT
TTAGTGGACCAACTGGCTCTGGCAAAAGTACTTTAATGTACCAAATGGTCCTTTATGCACATCAAAAATTGAATTTAAAT
GTAATATCAGTTGAAGATCCAGTTGAGCAAATACTTAATGGAATCACACAAATATCAGTCAATGAAAAGGCAGGTATAAA
TTATGAAAGCTCTTTCAAAGCCATTTTAAGGTGTGATCCAGATGTAATTTTAATTGGTGAAATCAGAGATTCTACCGTAG
CTAAATACGTGATTCAAGCCAGTTTAAGCGGGCATTTAGTTTTGTCGACAATGCACGCGAATGATTGCAAGGGAGCACTA
TTACGATTATTAGAAATGGGTATTTCTGTCCAAGAGTTGTGCCAGGCAATCAATTTAATTTCTAATCAAAGATTAATCAC
TACTACTACAAATTATCGTCAACTAGTATCGGAATTAATGTTTCAAAGCCAAATAAATTATTTTTTTGAACATAATCATT
CTTTACCTAAAAATTTCACAAAATTAGCTACACATTTAAACAAAATGTCTAAAGAAGGTGTTATATGTGAAGAAGTTGTC
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

70.062

100

0.701
  comGA Staphylococcus aureus N315

70.062

100

0.701