NTDB

Overview

Name	agrA	Type	Regulator
Locus tag	HWI39_RS05115	Genome accession	NZ_CP064543
Coordinates	1058657..1059373 (-)	Length	238 a.a.
NCBI ID	WP_001829999.1	Uniprot ID	C0LF82
Organism	Staphylococcus epidermidis strain UMCG366
Function	promote expression of competence genes (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	1050489..1076058	1058657..1059373	within	0

Gene organization within MGE regions

Location: 1050489..1076058

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HWI39_RS05080 (HWI39_05060)	-	1050489..1052027 (+)	1539	WP_001260014.1	recombinase family protein	-
HWI39_RS05085 (HWI39_05065)	-	1052102..1052857 (+)	756	Protein_999	YeeE/YedE family protein	-
HWI39_RS05090 (HWI39_05070)	-	1052909..1053133 (+)	225	WP_001830019.1	sulfurtransferase TusA family protein	-
HWI39_RS05095 (HWI39_05075)	-	1053553..1054803 (+)	1251	WP_001830014.1	ammonium transporter	-
HWI39_RS05100 (HWI39_05080)	-	1054938..1055891 (+)	954	WP_002457062.1	LacI family DNA-binding transcriptional regulator	-
HWI39_RS05105 (HWI39_05085)	-	1056151..1057623 (+)	1473	WP_001830011.1	sucrose-6-phosphate hydrolase	-
HWI39_RS05110 (HWI39_05090)	-	1057630..1058589 (+)	960	WP_001830023.1	carbohydrate kinase	-
HWI39_RS05115 (HWI39_05095)	agrA	1058657..1059373 (-)	717	WP_001829999.1	quorum-sensing response regulator AgrA	Regulator
HWI39_RS05120 (HWI39_05100)	agrC	1059390..1060319 (-)	930	Protein_1006	quorum-sensing sensor histidine kinase AgrC	-
HWI39_RS05125 (HWI39_05105)	-	1060414..1061586 (+)	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
HWI39_RS05130	-	1061634..1062011 (-)	378	Protein_1008	sensor histidine kinase AgrC	-
HWI39_RS05135 (HWI39_05110)	agrD	1062038..1062178 (-)	141	WP_001830021.1	cyclic lactone autoinducer peptide AgrD	-
HWI39_RS05140 (HWI39_05115)	-	1062162..1062746 (-)	585	WP_001830005.1	accessory gene regulator AgrB	-
HWI39_RS05145 (HWI39_05120)	hld	1063064..1063141 (+)	78	WP_002494082.1	delta-hemolysin	-
HWI39_RS05150 (HWI39_05125)	-	1063522..1064307 (-)	786	WP_001830000.1	carbon-nitrogen family hydrolase	-
HWI39_RS05155 (HWI39_05130)	sdrH	1064659..1065987 (+)	1329	WP_002468999.1	MSCRAMM-like protein SdrH	-
HWI39_RS05160 (HWI39_05135)	mroQ	1066047..1066787 (-)	741	WP_001830367.1	intramembrane glutamic endopeptidase MroQ	-
HWI39_RS05165 (HWI39_05140)	groES	1066962..1067246 (+)	285	WP_001830378.1	co-chaperone GroES	-
HWI39_RS05170 (HWI39_05145)	groL	1067302..1068921 (+)	1620	WP_001830447.1	chaperonin GroEL	-
HWI39_RS05175 (HWI39_05150)	-	1069036..1070211 (-)	1176	WP_049375261.1	site-specific integrase	-
HWI39_RS05180 (HWI39_05155)	-	1070220..1070747 (-)	528	WP_049375263.1	helix-turn-helix domain-containing protein	-
HWI39_RS05185 (HWI39_05160)	-	1070871..1071098 (+)	228	WP_049375265.1	helix-turn-helix domain-containing protein	-
HWI39_RS05190 (HWI39_05165)	-	1071102..1071725 (+)	624	WP_049375267.1	Bro-N domain-containing protein	-
HWI39_RS05195 (HWI39_05170)	-	1071739..1072053 (+)	315	WP_049375269.1	helix-turn-helix domain-containing protein	-
HWI39_RS05200 (HWI39_05175)	-	1072066..1072275 (+)	210	WP_049375271.1	hypothetical protein	-
HWI39_RS05205 (HWI39_05180)	-	1072279..1072683 (+)	405	WP_049375274.1	hypothetical protein	-
HWI39_RS05210 (HWI39_05185)	-	1072688..1072894 (+)	207	WP_002467971.1	hypothetical protein	-
HWI39_RS05215 (HWI39_05190)	tscT	1072909..1073214 (+)	306	WP_049375276.1	type II toxin-antitoxin system toxin TscT	-
HWI39_RS05220 (HWI39_05195)	-	1073274..1074875 (+)	1602	WP_049375278.1	hypothetical protein	-
HWI39_RS05225 (HWI39_05200)	-	1075255..1076058 (+)	804	WP_346415539.1	bifunctional DNA primase/polymerase	-

Sequence

Protein

Download Length: 238 a.a. Molecular weight: 27936.97 Da Isoelectric Point: 5.6250

                                >NTDB_id=502887 HWI39_RS05115 WP_001829999.1 1058657..1059373(-) (agrA) [Staphylococcus epidermidis strain UMCG366]

MKIFVCEDDQRQREHMVSIIKNYIMIEEKPMELALATNDPYEVLEQSKELNDIGCYFLDIQLEADMNGIKLASEIRKHDP

VGNIIFVTSHSELTYLTFVYKVAAMDFIFKDDPSELKMRIIDCLETAHTRLKLLSKESNVDTIELKRGSNSVYVQYDDIM

FFESSTKSHRLIAHLDNRQIEFYGNLKELAQLDERFFRCHNSFVINRHNIESIDSKERIVYFKNGENCFASVRNVKKI

Nucleotide

Download Length: 717 bp

                                >NTDB_id=502887 HWI39_RS05115 WP_001829999.1 1058657..1059373(-) (agrA) [Staphylococcus epidermidis strain UMCG366]

ATGAAAATTTTTGTTTGTGAAGATGACCAAAGACAAAGAGAACATATGGTATCAATCATTAAAAACTACATAATGATTGA

AGAAAAGCCAATGGAGTTAGCCTTAGCAACAAATGATCCTTATGAGGTCTTAGAGCAATCAAAAGAACTTAATGACATTG

GTTGTTACTTCCTTGATATTCAATTAGAAGCTGATATGAACGGTATTAAATTAGCCAGTGAAATTCGTAAACATGATCCT

GTTGGTAATATTATATTTGTAACCAGTCACAGTGAACTGACTTATTTGACGTTTGTTTATAAAGTGGCTGCTATGGATTT

TATTTTTAAGGATGATCCATCTGAATTAAAAATGAGAATCATAGATTGTCTTGAAACAGCACATACACGACTCAAATTAT

TATCAAAAGAAAGTAATGTAGATACGATTGAGTTAAAGCGGGGAAGTAATTCAGTATACGTTCAATATGATGACATTATG

TTTTTTGAATCATCTACGAAATCTCATAGACTCATCGCACATCTTGATAATCGACAAATTGAATTTTATGGAAATTTAAA

GGAATTAGCACAGCTTGATGAACGTTTCTTTAGATGTCATAACAGTTTTGTGATAAACAGGCATAATATTGAATCTATTG

ATTCAAAAGAACGTATTGTTTACTTTAAGAATGGCGAAAATTGTTTCGCTTCAGTACGTAATGTTAAAAAAATATAA

Domains

Predicted by InterproScan.

LytTr DNA-binding domain (LytTR)

(151-237)

Response regulator receiver domain (Response_reg)

(3-120)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	C0LF82

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
agrA	Staphylococcus aureus N315	87.815	100	0.878
comE/comE2	Streptococcus gordonii strain NCTC7865	35.178	100	0.374
comE/comE1	Streptococcus gordonii str. Challis substr. CH1	35.178	100	0.374

Detailed information NTG0502887