Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   HWI63_RS00185 Genome accession   NZ_CP064461
Coordinates   43466..45097 (-) Length   543 a.a.
NCBI ID   WP_002505023.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG391     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 43762..76708 43466..45097 flank -1335


Gene organization within MGE regions


Location: 43466..76708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI63_RS00185 (HWI63_00185) ccrB 43466..45097 (-) 1632 WP_002505023.1 cassette chromosome recombinase CcrB Machinery gene
  HWI63_RS00190 (HWI63_00190) ccrA 45094..46455 (-) 1362 WP_408020944.1 cassette chromosome recombinase CcrA Machinery gene
  HWI63_RS00195 (HWI63_00195) cch1 46642..48411 (-) 1770 WP_408020945.1 cassette chromosome replicative helicase -
  HWI63_RS00200 (HWI63_00200) ssb 48411..48701 (-) 291 WP_408020946.1 cassette chromosome ssDNA-binding protein -
  HWI63_RS00205 (HWI63_00205) - 48872..49942 (+) 1071 WP_271436914.1 hypothetical protein -
  HWI63_RS00210 (HWI63_00210) - 50036..51976 (+) 1941 WP_408020947.1 ATP-dependent DNA helicase -
  HWI63_RS00215 (HWI63_00215) - 52077..52541 (+) 465 WP_023604765.1 hypothetical protein -
  HWI63_RS00220 (HWI63_00220) - 52635..52784 (-) 150 WP_154391900.1 hypothetical protein -
  HWI63_RS00225 (HWI63_00225) - 53127..53765 (+) 639 WP_001033157.1 fusidic acid resistance EF-G-binding protein FusC -
  HWI63_RS00230 (HWI63_00230) - 54241..55382 (+) 1142 WP_145449897.1 IS3 family transposase -
  HWI63_RS00235 (HWI63_00235) - 55529..55957 (-) 429 WP_001011132.1 VOC family protein -
  HWI63_RS00240 (HWI63_00240) - 56037..56966 (+) 930 WP_000750310.1 YafY family protein -
  HWI63_RS00245 (HWI63_00245) - 57403..58659 (+) 1257 WP_000566670.1 MrcB family domain-containing protein -
  HWI63_RS00250 (HWI63_00250) - 59074..59313 (+) 240 WP_001282728.1 hypothetical protein -
  HWI63_RS00255 (HWI63_00255) - 59345..60019 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI63_RS00260 (HWI63_00260) - 60110..61093 (-) 984 Protein_49 protein rep -
  HWI63_RS00265 (HWI63_00265) mobV 61338..62600 (-) 1263 WP_000119405.1 MobV family relaxase -
  HWI63_RS00270 (HWI63_00270) bleO 63107..63511 (-) 405 WP_001242578.1 bleomycin binding protein -
  HWI63_RS00275 (HWI63_00275) aadD1 63728..64498 (-) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  HWI63_RS00280 (HWI63_00280) - 64691..65365 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI63_RS00285 (HWI63_00285) - 65630..66373 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  HWI63_RS00290 (HWI63_00290) - 66470..66898 (+) 429 WP_000872606.1 MaoC family dehydratase -
  HWI63_RS00295 (HWI63_00295) mecA 66944..68953 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HWI63_RS00300 (HWI63_00300) - 69050..70036 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  HWI63_RS00305 (HWI63_00305) - 70026..70268 (+) 243 Protein_58 type I restriction enzyme endonuclease domain-containing protein -
  HWI63_RS00310 (HWI63_00310) - 70259..71869 (-) 1611 Protein_59 IS1182 family transposase -
  HWI63_RS00315 (HWI63_00315) - 71918..72424 (-) 507 WP_001092194.1 DUF1643 domain-containing protein -
  HWI63_RS00320 (HWI63_00320) - 72439..72750 (-) 312 WP_000700851.1 DUF960 family protein -
  HWI63_RS00325 (HWI63_00325) - 72837..73187 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI63_RS00330 (HWI63_00330) ccrB 73709..75337 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  HWI63_RS00335 (HWI63_00335) ccrA 75359..76708 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 543 a.a.        Molecular weight: 62588.86 Da        Isoelectric Point: 9.6686

>NTDB_id=502034 HWI63_RS00185 WP_002505023.1 43466..45097(-) (ccrB) [Staphylococcus epidermidis strain UMCG391]
MTQLKKKRIGLYARVSTEIQTNGYSIQSQLNQLKEYCQFQGYEVVDEYTDRGISGKTTQRPELQRMLKDANDGKLDCIMV
YKTNRLARNTSDLLTIVEELYKINVEFFSLTEKIEIASSTGKLMLQILASFSEFERNTIVENVYNGQRQRAIEGYYQGNL
PLGYDKVPDSKKELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYIISNPFYIGKIQFAKY
RHWSDKRRKGLNEEPIIADGKHAPIIDKALWDKVQFKRQESRKKPQVHGKGTNLLTGIVKCPSPKCGAAMAASNTTNTLK
DGTKKRIRYYSCSNFRNKGSKVCSANSVRADVLEKYVMDQILEIIKSKKVLKQLVEKVNERSQIDVSSLNHDIAYKQSQC
EELKIKMHTLTKTIEDSPDLDSILKPTILNYQDELNQINNQIHQLEQDKQAEAPHYDADMIANILQTIFKDIDKLEKSQL
KSLYLTVIDRIDIRKDEHHKKQFYVTLKLNNEIIKQLFNNHPLDEVLLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1632 bp        

>NTDB_id=502034 HWI63_RS00185 WP_002505023.1 43466..45097(-) (ccrB) [Staphylococcus epidermidis strain UMCG391]
ATGACACAACTCAAAAAGAAACGTATTGGATTATATGCCCGTGTATCGACTGAAATCCAGACGAACGGCTACAGCATTCA
AAGCCAGTTAAATCAACTTAAAGAGTATTGTCAATTTCAAGGATATGAAGTCGTTGATGAATATACCGATCGAGGTATAT
CGGGTAAAACCACGCAACGTCCTGAACTACAACGTATGTTAAAAGATGCAAACGACGGTAAGTTAGATTGTATTATGGTC
TACAAAACCAATCGTTTAGCTAGAAATACCTCAGATTTATTAACTATTGTCGAAGAATTATATAAGATTAATGTCGAATT
TTTTAGTCTAACTGAAAAAATAGAAATTGCCAGCTCAACTGGTAAATTAATGCTCCAAATCTTAGCTAGCTTCTCTGAAT
TTGAAAGAAATACAATTGTAGAAAATGTCTACAATGGCCAAAGACAACGTGCTATTGAAGGTTATTACCAAGGTAATTTA
CCTCTAGGCTATGATAAAGTGCCTGATAGTAAAAAAGAACTGATGATTAATCAACATGAAGCAAATATTGTTAAATATAT
TTTTGAGTCATACGCCAAAGGGCACGGTTATCGTAAAATTGCGAATGCATTAAATCACAAAGGTTACGTGACTAAGAAAG
GGAAACCGTTTAGTATTTCATCCATTACCTATATCATCTCAAACCCTTTTTATATTGGAAAGATACAGTTTGCGAAGTAT
AGACACTGGAGCGATAAGAGACGAAAAGGACTCAATGAAGAACCGATTATTGCGGATGGTAAGCATGCGCCTATTATTGA
TAAAGCCTTATGGGATAAAGTACAGTTTAAACGCCAAGAGAGTCGTAAGAAACCACAAGTTCATGGTAAAGGAACCAATC
TACTGACTGGGATTGTAAAATGTCCGAGTCCGAAATGTGGCGCAGCAATGGCCGCAAGTAATACAACTAATACTTTAAAA
GATGGCACTAAGAAACGAATCAGATATTACTCATGTAGTAATTTTAGAAACAAAGGTTCTAAAGTATGTTCAGCCAATAG
TGTTAGAGCAGATGTATTGGAAAAATATGTTATGGATCAAATATTGGAGATTATCAAAAGTAAAAAGGTATTGAAACAAT
TGGTTGAAAAAGTAAATGAAAGAAGTCAAATAGACGTCTCTTCTTTGAATCATGATATTGCCTATAAACAGTCACAATGT
GAAGAACTAAAAATCAAAATGCATACACTAACGAAAACGATTGAAGACTCACCTGACTTAGATAGTATATTAAAACCAAC
AATACTTAACTATCAAGATGAATTGAATCAAATAAATAATCAAATCCATCAACTAGAACAAGATAAACAAGCGGAAGCTC
CACATTATGATGCTGATATGATTGCCAACATACTACAAACAATATTCAAAGATATAGACAAATTAGAGAAATCTCAATTG
AAATCACTATATCTAACAGTCATCGATCGCATTGACATCAGAAAAGATGAGCATCATAAGAAGCAATTTTATGTTACGCT
TAAACTGAATAATGAAATTATCAAACAACTATTTAATAATCATCCACTTGACGAAGTGCTCCTTAGCACTTCGTCTTTAT
TTTTGCCACAAACACTATATCTTACTATTTAA

Domains

Predicted by InterproScan.

Ogr/Delta-like zinc finger (Ogr_Delta)

(304-357)

Resolvase, N terminal domain (Resolvase)

(11-154)

Recombinase (Recombinase)

(179-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

73.162

100

0.733