Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   HWI63_RS00335 Genome accession   NZ_CP064461
Coordinates   75359..76708 (-) Length   449 a.a.
NCBI ID   WP_000815640.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG391     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 59377..89305 75359..76708 within 0
SCCmec 43762..76708 75359..76708 within 0


Gene organization within MGE regions


Location: 43762..89305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI63_RS00190 (HWI63_00190) ccrA 45094..46455 (-) 1362 WP_408020944.1 cassette chromosome recombinase CcrA Machinery gene
  HWI63_RS00195 (HWI63_00195) cch1 46642..48411 (-) 1770 WP_408020945.1 cassette chromosome replicative helicase -
  HWI63_RS00200 (HWI63_00200) ssb 48411..48701 (-) 291 WP_408020946.1 cassette chromosome ssDNA-binding protein -
  HWI63_RS00205 (HWI63_00205) - 48872..49942 (+) 1071 WP_271436914.1 hypothetical protein -
  HWI63_RS00210 (HWI63_00210) - 50036..51976 (+) 1941 WP_408020947.1 ATP-dependent DNA helicase -
  HWI63_RS00215 (HWI63_00215) - 52077..52541 (+) 465 WP_023604765.1 hypothetical protein -
  HWI63_RS00220 (HWI63_00220) - 52635..52784 (-) 150 WP_154391900.1 hypothetical protein -
  HWI63_RS00225 (HWI63_00225) - 53127..53765 (+) 639 WP_001033157.1 fusidic acid resistance EF-G-binding protein FusC -
  HWI63_RS00230 (HWI63_00230) - 54241..55382 (+) 1142 WP_145449897.1 IS3 family transposase -
  HWI63_RS00235 (HWI63_00235) - 55529..55957 (-) 429 WP_001011132.1 VOC family protein -
  HWI63_RS00240 (HWI63_00240) - 56037..56966 (+) 930 WP_000750310.1 YafY family protein -
  HWI63_RS00245 (HWI63_00245) - 57403..58659 (+) 1257 WP_000566670.1 MrcB family domain-containing protein -
  HWI63_RS00250 (HWI63_00250) - 59074..59313 (+) 240 WP_001282728.1 hypothetical protein -
  HWI63_RS00255 (HWI63_00255) - 59345..60019 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI63_RS00260 (HWI63_00260) - 60110..61093 (-) 984 Protein_49 protein rep -
  HWI63_RS00265 (HWI63_00265) mobV 61338..62600 (-) 1263 WP_000119405.1 MobV family relaxase -
  HWI63_RS00270 (HWI63_00270) bleO 63107..63511 (-) 405 WP_001242578.1 bleomycin binding protein -
  HWI63_RS00275 (HWI63_00275) aadD1 63728..64498 (-) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  HWI63_RS00280 (HWI63_00280) - 64691..65365 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI63_RS00285 (HWI63_00285) - 65630..66373 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  HWI63_RS00290 (HWI63_00290) - 66470..66898 (+) 429 WP_000872606.1 MaoC family dehydratase -
  HWI63_RS00295 (HWI63_00295) mecA 66944..68953 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HWI63_RS00300 (HWI63_00300) - 69050..70036 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  HWI63_RS00305 (HWI63_00305) - 70026..70268 (+) 243 Protein_58 type I restriction enzyme endonuclease domain-containing protein -
  HWI63_RS00310 (HWI63_00310) - 70259..71869 (-) 1611 Protein_59 IS1182 family transposase -
  HWI63_RS00315 (HWI63_00315) - 71918..72424 (-) 507 WP_001092194.1 DUF1643 domain-containing protein -
  HWI63_RS00320 (HWI63_00320) - 72439..72750 (-) 312 WP_000700851.1 DUF960 family protein -
  HWI63_RS00325 (HWI63_00325) - 72837..73187 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI63_RS00330 (HWI63_00330) ccrB 73709..75337 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  HWI63_RS00335 (HWI63_00335) ccrA 75359..76708 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  HWI63_RS00340 (HWI63_00340) cch1 76942..78735 (-) 1794 WP_000079473.1 cassette chromosome replicative helicase -
  HWI63_RS00345 (HWI63_00345) ssb 78735..79031 (-) 297 WP_408020948.1 cassette chromosome ssDNA-binding protein -
  HWI63_RS00350 (HWI63_00350) - 79203..80786 (+) 1584 WP_408020949.1 hypothetical protein -
  HWI63_RS00355 (HWI63_00355) - 81062..81415 (-) 354 WP_000278595.1 PTS glucitol/sorbitol transporter subunit IIA -
  HWI63_RS00360 (HWI63_00360) - 81431..82456 (-) 1026 WP_001066642.1 PTS glucitol/sorbitol transporter subunit IIB -
  HWI63_RS00365 (HWI63_00365) - 82468..83019 (-) 552 WP_000455774.1 PTS glucitol/sorbitol transporter subunit IIC -
  HWI63_RS00370 (HWI63_00370) - 83003..83476 (-) 474 WP_000897589.1 transcriptional regulator GutM -
  HWI63_RS00375 (HWI63_00375) - 83478..85214 (-) 1737 WP_225973925.1 PRD domain-containing protein -
  HWI63_RS00380 (HWI63_00380) - 85387..86181 (-) 795 WP_408020950.1 SDR family oxidoreductase -
  HWI63_RS00385 - 86971..87192 (+) 222 WP_020808100.1 hypothetical protein -
  HWI63_RS00390 - 87212..87457 (+) 246 WP_002502961.1 hypothetical protein -
  HWI63_RS00395 - 87459..87608 (+) 150 WP_001808882.1 hypothetical protein -
  HWI63_RS00400 (HWI63_00390) - 87904..88230 (+) 327 WP_408020951.1 helix-turn-helix domain-containing protein -
  HWI63_RS00405 (HWI63_00395) - 88267..89085 (+) 819 WP_002484875.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52663.34 Da        Isoelectric Point: 9.9639

>NTDB_id=502037 HWI63_RS00335 WP_000815640.1 75359..76708(-) (ccrA) [Staphylococcus epidermidis strain UMCG391]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=502037 HWI63_RS00335 WP_000815640.1 75359..76708(-) (ccrA) [Staphylococcus epidermidis strain UMCG391]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(169-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.947

100

0.759