Detailed information    

experimental Experimentally validated

Overview

Name   ccrB   Type   Machinery gene
Locus tag   SA_RS00430 Genome accession   NC_002745
Coordinates   63397..65025 (-) Length   542 a.a.
NCBI ID   WP_001186602.1    Uniprot ID   Q9XB94
Organism   Staphylococcus aureus N315     
Function   promote SCCmec transfer   
Homologous recombination

Function

SCCmec carries the ccr genes which encode recombinases that allow its excision and integration at the attB attachment site in the recipient's chromosome.


Genomic Context

Location: 58397..70025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA_RS00405 (SA0052) - 60065..61150 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  SA_RS00410 (SA0053) - 61269..61586 (-) 318 WP_000659050.1 JAB domain-containing protein -
  SA_RS00415 (SA0054) - 61607..62113 (-) 507 WP_001092169.1 DUF1643 domain-containing protein -
  SA_RS00420 (SA0055) - 62131..62442 (-) 312 WP_000700853.1 DUF960 family protein -
  SA_RS00425 (SA0056) - 62529..62879 (-) 351 WP_000859155.1 SAUGI family uracil-DNA glycosylase inhibitor -
  SA_RS00430 (SA0057) ccrB 63397..65025 (-) 1629 WP_001186602.1 cassette chromosome recombinase CcrB Machinery gene
  SA_RS00435 (SA0058) ccrA 65047..66396 (-) 1350 WP_000815646.1 cassette chromosome recombinase CcrA Machinery gene
  SA_RS00440 (SA0059) cch1 66630..68423 (-) 1794 WP_000195100.1 cassette chromosome replicative helicase -
  SA_RS00445 (SA0060) ssb 68423..68719 (-) 297 WP_000273824.1 cassette chromosome ssDNA-binding protein -
  SA_RS00450 (SA0061) - 68912..69958 (-) 1047 WP_001060127.1 hypothetical protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62664.84 Da        Isoelectric Point: 9.8600

>NTDB_id=11 SA_RS00430 WP_001186602.1 63397..65025(-) (ccrB) [Staphylococcus aureus N315]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRILKDAKEGYIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPNSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKLNNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKPLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=11 SA_RS00430 WP_001186602.1 63397..65025(-) (ccrB) [Staphylococcus aureus N315]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAATCGACCTGAGCTCCAACGTATATTGAAGGATGCGAAAGAAGGCTATATCGACTGTGTTATG
GTCTACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTATCAGAGCGTATGGAAGTCAATACTTCATCGGGTAAGCTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCTAATAGTAAACATGAACTGATGATTAATCAACATGAAGCTAATATTGTGAAATA
TATATTCGAGTCCTATGCCAAAGGCCATGGCTATCGTAAAATAGCCAATGCATTAAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCTATCACATATATATTAGCTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTTAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACACTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAACTAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGCCTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATAAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTGTTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterProScan.

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Mais Maree et al. (2024) SCCmec transformation requires living donor cells in mixed biofilms. Biofilm 7:100184. [PMID: 38440091]
[2] Mais Maree et al. (2022) Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms. Nature Communications 13(1):2477. [PMID: 35513365]